Anti-STAT3 (Phospho-Y705) Antibody (Clone#IFA-19)

Rabbit Monoclonal antibody

说明书

筛选器： All WB IHC ICC/IF IP

BM4835

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Figure 1. Western blot analysis of anti-STAT3 antibody (BM4835). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: mouse NIH/3T3 whole cell lysates,
Lane 3: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-STAT3 antigen affinity purified monoclonal antibody (BM4835) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for STAT3 at approximately 88 kDa. The expected band size for STAT3 is at 88 kDa.
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All lanes use the Antibody for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human pancreas, using Phospho-STAT3 (Y705) Antibody.
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Immunofluorescent analysis of HeLa cells treated with IFN-alpha, using Phospho-STAT3 (Y705) Antibody.
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产品简介>

产品名称

Anti-STAT3 (Phospho-Y705) Antibody (Clone#IFA-19)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Acute phase response factor; APRF; HIES; STAT3

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, IP

基因名称

STAT3

克隆号

IFA-19

抗体来源

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human STAT3

计算分子量

88 kDa

实际分子量

88 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

The transcription factor, signal transducer and activator of transcription-3 (STAT-3) is the most pleiotropic member of the signal transducer and activator of transcription (STAT) family of transcription factors and mediates pivotal responses for the cytokine family. The mouse STAT3 gene contains 24 exons and spans 30 kb. The translation initiation codon is in exon 2, and the stop codon is in exon 24. STAT3 is mapped to 17q21. It contributes to various physiological processes. Hepatic STAT-3 signaling is thus essential for normal glucose homeostasis and may provide new therapeutic targets for diabetes mellitus.

Uniprot ID

P40763

文献引用格式

STAT3 (Phospho-Y705) Antibody (Clone#IFA-19) (Boster Biological Technology, Wuhan, China. Catalog#BM4835)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Signal transducer and activator of transcription 3

实验方案>

实验步骤

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