Figure 1. Western blot analysis of anti-STAT3 (Phospho-S727) antibody (BM4169). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human U251 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-STAT3 (Phospho-S727) antigen affinity purified monoclonal antibody (BM4169) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for STAT3 (Phospho-S727) at approximately 88 kDa. The expected band size for STAT3 (Phospho-S727) is at 88 kDa.
Figure 2. IF analysis of STAT3 using anti-STAT3 antibody (BM4169).
STAT3 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-STAT3 Antibody (BM4169) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Figure 2. IF analysis of STAT3 using anti-STAT3 antibody (BM4169).
STAT3 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-STAT3 Antibody (BM4169) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-200 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-200 |
| ImmunoPrecipitation (IP): | 1:20 |
Figure 1. Western blot analysis of anti-STAT3 (Phospho-S727) antibody (BM4169). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human U251 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-STAT3 (Phospho-S727) antigen affinity purified monoclonal antibody (BM4169) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for STAT3 (Phospho-S727) at approximately 88 kDa. The expected band size for STAT3 (Phospho-S727) is at 88 kDa.
Figure 2. IF analysis of STAT3 using anti-STAT3 antibody (BM4169).
STAT3 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-STAT3 Antibody (BM4169) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Figure 2. IF analysis of STAT3 using anti-STAT3 antibody (BM4169).
STAT3 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-STAT3 Antibody (BM4169) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Figure 1. Western blot analysis of anti-STAT3 (Phospho-S727) antibody (BM4169). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human U251 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-STAT3 (Phospho-S727) antigen affinity purified monoclonal antibody (BM4169) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for STAT3 (Phospho-S727) at approximately 88 kDa. The expected band size for STAT3 (Phospho-S727) is at 88 kDa.
Figure 2. IF analysis of STAT3 using anti-STAT3 antibody (BM4169).
STAT3 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-STAT3 Antibody (BM4169) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Figure 2. IF analysis of STAT3 using anti-STAT3 antibody (BM4169).
STAT3 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-STAT3 Antibody (BM4169) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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