Anti-P27/KIP1/CDKN1B Antibody (Clone#CGA-3)

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF IP FCM

BM4229

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Western blot analysis of anti-P27/KIP1/CDKN1B antibody (BM4229). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-P27/KIP1/CDKN1B antigen affinity purified monoclonal antibody (BM4229) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for P27/KIP1/CDKN1B at approximately 25 kDa. The expected band size for P27/KIP1/CDKN1B is at 22 kDa.
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IHC analysis of P27/KIP1/CDKN1B using anti-P27/KIP1/CDKN1B antibody (BM4229) .
P27/KIP1/CDKN1B was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-P27/KIP1/CDKN1B Antibody (BM4229) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of P27/KIP1/CDKN1B using anti-P27/KIP1/CDKN1B antibody (BM4229) .
P27/KIP1/CDKN1B was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-P27/KIP1/CDKN1B Antibody (BM4229) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of P27/KIP1/CDKN1B using anti-P27/KIP1/CDKN1B antibody (BM4229) .
P27/KIP1/CDKN1B was detected in a paraffin-embedded section of human urothelial carcinoma with squamous differentiation tissue. The tissue section was incubated with rabbit anti-P27/KIP1/CDKN1B Antibody (BM4229) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of P27/KIP1/CDKN1B using anti-P27/KIP1/CDKN1B antibody (BM4229) .
P27/KIP1/CDKN1B was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-P27/KIP1/CDKN1B Antibody (BM4229) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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Immunofluorescent analysis of Hela cells, using p27 KIP 1 Antibody .
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产品简介>

产品名称

Anti-P27/KIP1/CDKN1B Antibody (Clone#CGA-3)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CDKN1B; CDKN4; KIP1; MEN1B; MEN4; P27; P27; KIP1; P27KIP1

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, IP, FCM

基因名称

CDKN1B

克隆号

CGA-3

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human p27 KIP 1

计算分子量

22 kDa

实际分子量

25 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

P27(kip1), also known as cyclin dependent kinase inhibitor 1B(CDKN1B), is a major target of AFX-like forkhead proteins. CDKN1B(p27) belongs to the Cip/Kip family and functions as an important cell cycle gatekeeper. Phosphorylation leads to the ubiquitination and degradation of CDKN1B. P27(kip1) mapped to 12p13. An increase in p27 causes proliferating cells to exit from the cell cycle, and a decrease in p27 is necessary for quiescent cells to resume division. Abnormally low amounts of p27 are associated with pathological states of excessive cell proliferation, especially cancers. Overexpression of p27Kip1 lengthens the G1 phase in a mouse model that targets inducible gene expression to central nervous system progenitor cells.

Uniprot ID

P46527

文献引用格式

P27/KIP1/CDKN1B Antibody (Clone#CGA-3) (Boster Biological Technology, Wuhan, China. Catalog#BM4229)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Cyclin-dependent kinase inhibitor 1B

实验方案>

实验步骤

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