Anti-P27/KIP1/CDKN1B Antibody

筛选器： All WB ICC/IF FCM

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  Western blot analysis of anti- CDKN1B antibody (PB0075). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human MCF-7 whole cell lysates.
  Use rabbit anti- CDKN1B 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for CDKN1B at approximately 27KD. The expected band size for CDKN1B is at 27KD.

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  IF analysis of P27/KIP1/CDKN1B using anti-P27/KIP1/CDKN1B antibody (PB0075).
  P27/KIP1/CDKN1B was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-P27/KIP1/CDKN1B Antibody (PB0075) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody.

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  Flow Cytometry analysis of SiHa cells using anti-P27/KIP1/CDKN1B antibody (PB0075).
  Overlay histogram showing SiHa cells stained with PB0075 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-P27/KIP1/CDKN1B Antibody (PB0075) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Western blot analysis of anti- CDKN1B antibody (PB0075). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human MCF-7 whole cell lysates.
  Use rabbit anti- CDKN1B 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for CDKN1B at approximately 27KD. The expected band size for CDKN1B is at 27KD.

• 

  IF analysis of P27/KIP1/CDKN1B using anti-P27/KIP1/CDKN1B antibody (PB0075).
  P27/KIP1/CDKN1B was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-P27/KIP1/CDKN1B Antibody (PB0075) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody.

• 

  Flow Cytometry analysis of SiHa cells using anti-P27/KIP1/CDKN1B antibody (PB0075).
  Overlay histogram showing SiHa cells stained with PB0075 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-P27/KIP1/CDKN1B Antibody (PB0075) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.