产品名称
Anti-Histone H4 (tri methyl K20) Antibody (Clone#DHB-8)
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
H4F2; HIST; HIST1H4A; Histone H4; H4K20me3
基因名称
H4C1/H4C2/H4C3/H4C4/H4C5/H4C6/H4C8/H4C9/H4C11/H4C12/H4C13/H4C14/H4C15/H4C16
免疫原
A synthesized peptide derived from human Histone H4 (tri methyl K20)
成分
500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.
纯化方式
Affinity-chromatography
保存条件
12 months from date of receipt，-20℃ as supplied.
背景资料
Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases.
文献引用格式
Histone H4 (tri methyl K20) Antibody (Clone#DHB-8) (Boster Biological Technology, Wuhan, China. Catalog#BM4343)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐稀释比
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-200 |
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