Anti-Histone H4 Antibody (Clone#HDC-8)

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF

BM4713

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-Histone H4 antibody (BM4713). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human U2OS whole cell lysates,
Lane 4: human Hacat whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse 3T3-L1 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Histone H4 antigen affinity purified monoclonal antibody (BM4713) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Histone H4 at approximately 11 kDa. The expected band size for Histone H4 is at 11 kDa.
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IHC analysis of Histone H4 using anti-Histone H4 antibody (BM4713) .
Histone H4 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-Histone H4 Antibody (BM4713) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(13)
IHC analysis of Histone H4 using anti-Histone H4 antibody (BM4713) .
Histone H4 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section was incubated with rabbit anti-Histone H4 Antibody (BM4713) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(13)
IHC analysis of Histone H4 using anti-Histone H4 antibody (BM4713) .
Histone H4 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-Histone H4 Antibody (BM4713) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of Histone H4 using anti-Histone H4 antibody (BM4713) .
Histone H4 was detected in a paraffin-embedded section of human lung squamous cell carcinoma tissue. The tissue section was incubated with rabbit anti-Histone H4 Antibody (BM4713) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen
all(13)
IHC analysis of Histone H4 using anti-Histone H4 antibody (BM4713) .
Histone H4 was detected in a paraffin-embedded section of human placenta tissue. The tissue section was incubated with rabbit anti-Histone H4 Antibody (BM4713) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(13)
IHC analysis of Histone H4 using anti-Histone H4 antibody (BM4713) .
Histone H4 was detected in a paraffin-embedded section of human testicular germ cell tumors tissue. The tissue section was incubated with rabbit anti-Histone H4 Antibody (BM4713) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(13)
IHC analysis of Histone H4 using anti-Histone H4 antibody (BM4713) .
Histone H4 was detected in a paraffin-embedded section of human thyroid cancer tissue. The tissue section was incubated with rabbit anti-Histone H4 Antibody (BM4713) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(13)
IHC analysis of Histone H4 using anti-Histone H4 antibody (BM4713) .
Histone H4 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-Histone H4 Antibody (BM4713) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(13)
IHC analysis of Histone H4 using anti-Histone H4 antibody (BM4713) .
Histone H4 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-Histone H4 Antibody (BM4713) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(13)
IHC analysis of Histone H4 using anti-Histone H4 antibody (BM4713) .
Histone H4 was detected in a paraffin-embedded section of human B-cell lymphoma tissue. The tissue section was incubated with rabbit anti-Histone H4 Antibody (BM4713) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(13)
IHC analysis of Histone H4 using anti-Histone H4 antibody (BM4713) .
Histone H4 was detected in a paraffin-embedded section of human colon tissue. The tissue section was incubated with rabbit anti-Histone H4 Antibody (BM4713) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-Histone H4 Antibody (Clone#HDC-8)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

Histone H4; HIST1H4A; H4/A; H4FA; HIST1H4B; H4/I; H4FI; HIST1H4C; H4/G; H4FG; HIST1H4D; H4/B; H4FB; HIST1H4E; H4/J; H4FJ; HIST1H4F; H4/C; H4FC; HIST1H4H; H4/H; H4FH; HIST1H4I; H4/M; H4FM; HIST1H4J; H4/E; H4FE; HIST1H4K; H4/D; H4FD; HIST1H4L; H4/K; H4FK

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF

基因名称

H4C1/H4C2/H4C3/H4C4/H4C5/H4C6/H4C8/H4C9/H4C11/H4C12/H4C13/H4C14/H4C15/H4C16

克隆号

HDC-8

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Histone H4

计算分子量

11 kDa

实际分子量

11 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases.

Uniprot ID

P62805

文献引用格式

Histone H4 Antibody (Clone#HDC-8) (Boster Biological Technology, Wuhan, China. Catalog#BM4713)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Histone H4

实验方案>

实验步骤

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