Anti-RUNX2 Antibody (Clone#HCO-18)

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF

BM4700

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-RUNX2 antibody (BM4700). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates,
Lane 2: human U251 whole cell lysates,
Lane 3: human RT4 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RUNX2 antigen affinity purified monoclonal antibody (BM4700) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for RUNX2 at approximately 57 kDa. The expected band size for RUNX2 is at 57 kDa.
all(3)
IHC analysis of RUNX2 using anti-RUNX2 antibody (BM4700) .
RUNX2 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-RUNX2 Antibody (BM4700) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(3)
Immunofluorescent analysis of Saos-2 cells, using RUNX2 Antibody.
all(3)

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产品简介>

产品名称

Anti-RUNX2 Antibody (Clone#HCO-18)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

AML3; CBF alpha 1; CBFA1; CCD; CCD1; Oncogene AML 3; OSF 2; OSF2; PEA2 alpha A; PEA2aA; PEBP2 alpha A; PEBP2A; PEBP2A1; PEBP2A2; PEBP2aA; PEBP2aA1; RUNX2

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF

基因名称

RUNX2

克隆号

HCO-18

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human RUNX2

计算分子量

57 kDa

实际分子量

57 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

RUNX2 regulates the transcription of various genes, including osteopontin, bone sialoprotein, and osteocalcin, via binding to the core site of the enhancers or promoters. RUNX2 is crucial for the maturation of osteoblasts and both intramembranous and endochondral ossification.

Uniprot ID

Q13950

文献引用格式

RUNX2 Antibody (Clone#HCO-18) (Boster Biological Technology, Wuhan, China. Catalog#BM4700)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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