Anti-RUNX2 Antibody (Clone#OTI3E12)

Mouse monoclonal antibody

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筛选器： All WB

MA00442

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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HEK293T cells were transfected with the RUNX2 transcript variant 1 (Left lane) or RUNX2 transcript variant 2 (Right lane) cDNA clone for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RUNX2 antibody(Cat# MA00442).
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RUNX2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RUNX2 antibody (Cat# MA00442)(1:1000)
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产品简介>

产品名称

Anti-RUNX2 Antibody (Clone#OTI3E12)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

AML3; CBF alpha 1; CBFA1; CCD; CCD1; Oncogene AML 3; OSF 2; OSF2; PEA2 alpha A; PEA2aA; PEBP2 alpha A; PEBP2A; PEBP2A1; PEBP2A2; PEBP2aA; PEBP2aA1; RUNX2

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

基因名称

RUNX2

克隆号

OTI3E12

宿主

Mouse

抗体亚型

IgG1

免疫原

Human recombinant protein fragment corresponding to amino acids 1-293 of human RUNX2 (NP_004339) produced in E.coli.

计算分子量

57 kDa

实际分子量

54.9 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.

背景资料

This gene is a member of the RUNX family of transcription factors and encodes a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Two regions of potential trinucleotide repeat expansions are present in the N-terminal region of the encoded protein, and these and other mutations in this gene have been associated with the bone development disorder cleidocranial dysplasia (CCD). Transcript variants that encode different protein isoforms result from the use of alternate promoters as well as alternate splicing. [provided by RefSeq, Jul 2016]

Uniprot ID

Q13950

文献引用格式

RUNX2 Antibody (Clone#OTI3E12) (Boster Biological Technology, Wuhan, China. Catalog#MA00442)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

实验方案>

实验步骤

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