Anti-RUNX2 Antibody

Rabbit polyclonal antibody

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筛选器： All WB ICC/IF

PB0171

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of RUNX2 using anti-RUNX2 antibody (PB0171).
Lane 1: recombinant human RUNX2 protein 0.5ng.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RUNX2 antigen affinity purified polyclonal antibody (PB0171) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for RUNX2 at approximately 50 kDa.
all(3)
Western blot analysis of RUNX2 using anti-RUNX2 antibody (PB0171). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HELA whole cell lysates,
Lane 2: A431 whole cell lysates,
Lane 3: K562 whole cell lysates,
Lane 4: JURKAT whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RUNX2 antigen affinity purified polyclonal antibody (PB0171) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for RUNX2 at approximately 57 kDa. The expected band size for RUNX2 is at 57 kDa.
all(3)
IF analysis of RUNX2 using anti-RUNX2 antibody (PB0171).
RUNX2 was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-RUNX2 Antibody (PB0171) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
all(3)

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产品简介>

产品名称

Anti-RUNX2 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

AML3; CBF alpha 1; CBFA1; CCD; CCD1; Oncogene AML 3; OSF 2; OSF2; PEA2 alpha A; PEA2aA; PEBP2 alpha A; PEBP2A; PEBP2A1; PEBP2A2; PEBP2aA; PEBP2aA1; RUNX2

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, ICC/IF

基因名称

RUNX2

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence in the middle region of human RUNX2, identical to the related mouse sequence.

计算分子量

57 kDa

实际分子量

57 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Core binding factor A1 (CBFA1/RUNX2) is a runt-like transcription factor essential for osteoblast differentiation. This protein is a member of the RUNX family of transcription factors and has a Runt DNA-binding domain. It is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. RUNX2 plays a non-redundant role for Cbfa1 in tooth development that may be distinct from that in bone formation. In odontogenesis, RUNX2 is not involved in the early signaling networks regulating tooth initiation and early morphogenesis but regulates key epithelial-mesenchymal interactions that control advancing morphogenesis and histodifferentiation of the epithelial enamel organ.

Uniprot ID

Q13950

文献引用格式

RUNX2 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB0171)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

runt-related transcription factor 2

蛋白名全称

Runt-related transcription factor 2

实验方案>

实验步骤

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