产品名称
Human Angiogenin/ANG ELISA Kit
指标别名
ALS9; ANG; Angiogenin; HEL168; Ribonuclease 5; RNase 5; RNASE4; RNASE5
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
78 pg/ml - 5000 pg/ml
基因名全称
angiogenin, ribonuclease, RNase A family, 5
文献引用格式
Human Angiogenin/ANG ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0305)
背景资料
Angiogenin(Ang) also known as ribonuclease 5 is a protein that in humans is encoded by the ANG gene. Angiogenin is a potent stimulator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. Hooper et al.(2003) reviewed the evidence that angiogenins are involved in host defense and noted that inflammation provokes upregulated ANG mRNA expression in liver and an increase in detectable ANG protein in serum. Weremowicz et al.(1989, 1990) assigned the human angiogenin gene to chromosome 14q11.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0305-CAP | 96-well plate precoated with anti-human ANG antibody | 1 |
EK0305-ST | Lyophilized recombinant human ANG standard | 10ng/tube×2 |
EK0305-DA | Biotinylated anti- human ANG antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 107 | 719 | 2914 | 100 | 723 | 2957 |
Standard deviation | 4.81 | 48.17 | 195.23 | 4.6 | 49.16 | 251.34 |
CV(%) | 4.5 | 6.7 | 6.7 | 4.6 | 6.8 | 8.5 |
典型数据示例
Concentration(pg/ml) | 0.0 78 | 156 | 313 | 625 | 1250 | 2500 | 5,000 |
O.D. | 0.047 | 0.116 | 0.169 | 0.265 | 0.506 | 0.927 | 1.534 | 2.375 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)