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Human Angiogenin/ANG ELISA Kit

说明书

EK0305

  • 96T ¥2480
  • 货期: 现货

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产品名称
Human Angiogenin/ANG ELISA Kit
规格/价格
96T/2480
指标别名
ALS9; ANG; Angiogenin; HEL168; Ribonuclease 5; RNase 5; RNASE4; RNASE5
免疫原
E.coli, Q25-P147
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
78 pg/ml - 5000 pg/ml
基因名称
ANG
基因名全称
angiogenin, ribonuclease, RNase A family, 5
Uniprot ID
P03950  
文献引用格式
Human Angiogenin/ANG ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0305)
背景资料
Angiogenin(Ang) also known as ribonuclease 5 is a protein that in humans is encoded by the ANG gene. Angiogenin is a potent stimulator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. Hooper et al.(2003) reviewed the evidence that angiogenins are involved in host defense and noted that inflammation provokes upregulated ANG mRNA expression in liver and an increase in detectable ANG protein in serum. Weremowicz et al.(1989, 1990) assigned the human angiogenin gene to chromosome 14q11.
显色时间
15-20分钟
试剂盒内容
Catalog numberDescriptionQuantity
EK0305-CAP96-well plate precoated with anti-human ANG antibody1
EK0305-STLyophilized recombinant human ANG standard10ng/tube×2
EK0305-DABiotinylated anti- human ANG antibody100μl(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex (ABC)100μl(dilution 1:100)
AR1106-1Sample diluent buffer30 ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Intra-Assay Precision
Inter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)10771929141007232957
Standard deviation4.8148.17195.234.649.16251.34
CV(%)4.56.76.74.66.88.5
典型数据示例
Concentration(pg/ml)0.0 78156313625125025005,000
O.D.0.0470.1160.1690.2650.5060.9271.5342.375
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
human
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)
实验时长
3.5h

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