产品名称
Human Angiogenin/ANG EZ-Set™ ELISA Kit (DIY Antibody Pairs)
指标别名
ALS9; ANG; Angiogenin; HEL168; Ribonuclease 5; RNase 5; RNASE4; RNASE5
免疫原
Expression system for standard: E.coli; Immunogen sequence: Q25-P147
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
78 pg/ml - 5,000 pg/ml
文献引用格式
Human Angiogenin/ANG EZ-Set™ ELISA Kit (DIY Antibody Pairs) (Boster Biological Technology, Wuhan, China. Catalog#EZ0305)
背景资料
Angiogenin(Ang) also known as ribonuclease 5 is a protein that in humans is encoded by the ANG gene. Angiogenin is a potent stimulator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. Hooper et al.(2003) reviewed the evidence that angiogenins are involved in host defense and noted that inflammation provokes upregulated ANG mRNA expression in liver and an increase in detectable ANG protein in serum. Weremowicz et al.(1989, 1990) assigned the human angiogenin gene to chromosome 14q11.
试剂盒内容
| Catalog number |
Description |
Quantity |
Storage of opened/reconstituted material |
| EZ0305-CA |
Rabbit anti- human ANG polyclonal antibody (Capture Antibody) |
500 µl, 0.4 mg/mL
(recommended dilution 1:100) |
Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit.
|
| EZ0305-DA |
Biotinylated goat anti- human ANG polyclonal antibody (Detection Antibody) |
500 µl, 8.3 µg/mL
(recommended dilution 1:100) |
| AR1103 |
Avidin-Biotin-Peroxidase Complex (ABC) |
500 µl (recommended dilution 1:100) |
| EZ0305-ST |
Lyophilized recombinant human ANG standard |
10 ng/tube×3
|
Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the
expiration date of the kit. |
典型数据示例
| Concentration (pg/ml) | 0 | 78 | 156 | 313 | 625 | 1250 | 2500 | 5000 |
| O.D. | 0.047 | 0.116 | 0.169 | 0.265 | 0.506 | 0.927 | 1.534 | 2.375 |
保存条件与有效期
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
需要自备的试剂
1. Microplate reader in standard size.
2. Automated plate washer.
3. Incubator.
4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
5. Clean tubes and Eppendorf tubes.
6. 96 well microplate (Cat# AR1100)
7. Plate Sealers.
8. Capture Antibody Diluent: PBS.
9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
10. Substrate Solution:
Tetramethylbenzidine (Cat# AR1104)
11. Stop Solution: 2 N H2SO4 (Cat# AR1105)
12.Wash Buffer (PBS and PBS-T). Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
*Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)
*样品准备的相关试剂也不含在试剂盒中。
样本类型
Cell Culture Supernatants, Cell Lysates, Tissue Homogenates, Serum and Plasma (heparin, EDTA)
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