产品名称
Human Angiogenin/ANG Quick ELISA Kit
指标别名
ALS9; ANG; Angiogenin; HEL168; Ribonuclease 5; RNase 5; RNASE4; RNASE5
免疫原
Expression system for standard: E.coli; Immunogen sequence: Q25-P147
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
angiogenin, ribonuclease, RNase A family, 5
文献引用格式
Human Angiogenin/ANG Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK0305)
背景资料
Angiogenin(Ang) also known as ribonuclease 5 is a protein that in humans is encoded by the ANG gene. Angiogenin is a potent stimulator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. Hooper et al.(2003) reviewed the evidence that angiogenins are involved in host defense and noted that inflammation provokes upregulated ANG mRNA expression in liver and an increase in detectable ANG protein in serum. Weremowicz et al.(1989, 1990) assigned the human angiogenin gene to chromosome 14q11.
试剂盒内容
Catalog number | Description | Quantity |
FEK0305-CAP | Anti-Pre-coated 96-well Strip Microplate | 1 |
FEK0305-ST | Human ANG standard | 10ng/tube×2 |
FEK0305-A | Human ANG Antibody A | 3ml |
FEK0305-B | Human ANG Antibody B | 3ml |
AR1106-1 | Sample diluent buffer | 15ml |
AR1106-7 | TBS-T Wash Buffer (25x) | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | Stop solution | 10ml |
PLA-SEA | Adhesive cover | 2 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 107 | 719 | 2914 | 100 | 723 | 2957 |
Standard deviation | 4.81 | 48.17 | 195.23 | 4.6 | 49.16 | 251.34 |
CV(%) | 4.5% | 6.7% | 6.7% | 4.6% | 6.8% | 8.5% |
典型数据示例
Concentration(pg/ml) | 0 | 78 | 156 | 313 | 625 | 1250 | 2500 | 5,000 |
O.D. | 0.035 | 0.060 | 0.177 | 0.398 | 0.795 | 1.363 | 1.996 | 2.429 |
保存条件与有效期
Store at 4℃ for 6 months.
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)