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Human ICAM1 ELISA Kit

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EK0370

  • 96T ¥2280
  • 货期: 现货

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产品名称
Human ICAM1 ELISA Kit
规格/价格
96T/2280
指标别名
BB2; CD54; ICAM 1; ICAM1; ICAM-1; P3.58; Ly 47; MALA 2
免疫原
NSO, Q28-E480
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
156 pg/ml - 10000 pg/ml
基因名称
ICAM1
基因名全称
intercellular adhesion molecule 1
Uniprot ID
P05362  
文献引用格式
Human ICAM1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0370)
背景资料
Intercellular adhesion molecule-1(ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for lymphocyte function-associated(LFA) antigens, a beta 2 leukocyte integrin. The normal function of human intercellular adhesion molecule-1(ICAM-1) is to provide adhesion between endothelial cells and leukocytes after injury or stress. ICAM-1 binds to leukocyte function-associated antigen(LFA-1) or macrophage-1 antigen(Mac-1). ICAM-1 is found on leukocytes, fibroblasts, epithelial cells and endothelial cells and its expression is regulated by inflammatory cytokines. ICAM-1 has a tissue distribution similar to that of the major histocompatibility complex class II antigens and is likely to play a role in inflammatory responses.1
显色时间
15-20分钟
试剂盒内容
Catalog numberDescriptionQuantity
EK0370-CAP96-well plate precoated with anti- human ICAM-1 antibody1
EK0370-STLyophilized recombinant human ICAM-1 standard10ng/tube×2
EK0370-DABiotinylated anti- human ICAM-1 antibody100μl(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex (ABC)100μl(dilution 1:100)
AR1106-1Sample diluent buffer30 ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Intra-Assay Precision
Inter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)2721923474624819294549
Standard deviation14.41111.53204.0714.13125.38236.54
CV(%)5.35.84.35.76.55.2
典型数据示例
Concentration(pg/ml)015631262512502500500010000
O.D.0.0060.0880.1870.4080.7421.3511.8842.392
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
human
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)
实验时长
3.5h

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