产品名称
Human ICAM1 ELISA Kit
指标别名
BB2; CD54; ICAM 1; ICAM1; ICAM-1; P3.58; Ly 47; MALA 2
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
156 pg/ml - 10000 pg/ml
基因名全称
intercellular adhesion molecule 1
文献引用格式
Human ICAM1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0370)
背景资料
Intercellular adhesion molecule-1(ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for lymphocyte function-associated(LFA) antigens, a beta 2 leukocyte integrin. The normal function of human intercellular adhesion molecule-1(ICAM-1) is to provide adhesion between endothelial cells and leukocytes after injury or stress. ICAM-1 binds to leukocyte function-associated antigen(LFA-1) or macrophage-1 antigen(Mac-1). ICAM-1 is found on leukocytes, fibroblasts, epithelial cells and endothelial cells and its expression is regulated by inflammatory cytokines. ICAM-1 has a tissue distribution similar to that of the major histocompatibility complex class II antigens and is likely to play a role in inflammatory responses.1
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0370-CAP | 96-well plate precoated with anti- human ICAM-1 antibody | 1 |
EK0370-ST | Lyophilized recombinant human ICAM-1 standard | 10ng/tube×2 |
EK0370-DA | Biotinylated anti- human ICAM-1 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 272 | 1923 | 4746 | 248 | 1929 | 4549 |
Standard deviation | 14.41 | 111.53 | 204.07 | 14.13 | 125.38 | 236.54 |
CV(%) | 5.3 | 5.8 | 4.3 | 5.7 | 6.5 | 5.2 |
典型数据示例
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.006 | 0.088 | 0.187 | 0.408 | 0.742 | 1.351 | 1.884 | 2.392 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)