Mouse ICAM1 ELISA Kit

96T/2280

BB2; CD54; ICAM 1; ICAM1; ICAM-1; P3.58; Ly 47; MALA 2

Expression system for standard: NS0; Immunogen sequence: Q28-N485

There is no detectable cross-reactivity with other relevant proteins.

156pg/ml-10000pg/ml

ICAM1

Intercellular adhesion molecule 1

P13597  

Mouse ICAM1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0371)

Intercellular adhesion molecule-1 (ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for lymphocyte function-associated (LFA) antigens, a beta 2 leukocyte integrin. The normal function of human intercellular adhesion molecule-1 (ICAM-1) is to provide adhesion between endothelial cells and leukocytes after injury or stress. ICAM-1 binds to leukocyte function-associated antigen (LFA-1) or macrophage-1 antigen (Mac-1). ICAM-1 is found on leukocytes, fibroblasts, epithelial cells and endothelial cells and its expression is regulated by inflammatory cytokines. ICAM-1 has a tissue distribution similar to that of the major histocompatibility complex class II antigens and is likely to play a role in inflammatory responses. The standard product used in this kit is produced by recombinant gene expression, which is a dimeric protein linked with disulfide bonds. The single chain is Gln28-Asn 485. It has 457 amino acids with the molecular mass of 76.8kDa. As a result of glycosylation, the molecular mass of 110-130KDa is revealed by SDS-PAGE.

Catalog number	Description	Quantity
EK0371-CAP	96-well plate precoated with anti- mouse ICAM-1 antibody	1
EK0371-ST	Lyophilized recombinant mouse ICAM-1 standard	10ng/tube×2
EK0371-DA	Biotinylated anti- mouse ICAM-1 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	354	1378	4978	361	1458	5187
Standard deviation	21.94	62.01	318.59	25.27	83.1	414.96
CV(%)	6.2%	4.5%	6.4%	7%	5.7%	8%

Concentration(pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.059	0.124	0.129	0.297	0.451	0.640	1.345	1.916

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

mouse

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Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)

3.5h