产品名称
Human ICAM1 Quick ELISA Kit
指标别名
BB2; CD54; ICAM 1; ICAM1; ICAM-1; P3.58; Ly 47; MALA 2
免疫原
Expression system for standard: NS0; Immunogen sequence: Q28-E480
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
intercellular adhesion molecule 1
文献引用格式
Human ICAM1 Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK0370)
背景资料
Intercellular adhesion molecule-1(ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for lymphocyte function-associated(LFA) antigens, a beta 2 leukocyte integrin. The normal function of human intercellular adhesion molecule-1(ICAM-1) is to provide adhesion between endothelial cells and leukocytes after injury or stress. ICAM-1 binds to leukocyte function-associated antigen(LFA-1) or macrophage-1 antigen(Mac-1). ICAM-1 is found on leukocytes, fibroblasts, epithelial cells and endothelial cells and its expression is regulated by inflammatory cytokines. ICAM-1 has a tissue distribution similar to that of the major histocompatibility complex class II antigens and is likely to play a role in inflammatory responses.1
试剂盒内容
Catalog number | Description | Quantity |
FEK0370-CAP | Anti-Pre-coated 96-well Strip Microplate | 1 |
FEK0370-ST | Human ICAM-1 standard | 10ng/tube×2 |
FEK0370-A | Human ICAM-1 Antibody A | 3ml |
FEK0370-B | Human ICAM-1 Antibody B | 3ml |
AR1106-1 | Sample diluent buffer | 15ml |
AR1106-7 | TBS-T Wash Buffer (25x) | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | Stop solution | 10ml |
PLA-SEA | Adhesive cover | 2 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 272 | 1923 | 4746 | 248 | 1929 | 4549 |
Standard deviation | 14.41 | 111.53 | 204.07 | 14.13 | 125.38 | 236.54 |
CV(%) | 5.3% | 5.8% | 4.3% | 5.7% | 6.5% | 5.2% |
典型数据示例
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.061 | 0.113 | 0.159 | 0.212 | 0.330 | 0.571 | 1.027 | 1.832 |
保存条件与有效期
Store at 4℃ for 6 months.
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)