Human IL12(p70) ELISA Kit

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EK0421

96T ￥2280
货期：现货

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产品名称

Human IL12(p70) ELISA Kit

规格/价格

96T/2280

指标别名

CLMF; CLMF p35; IL 12 subunit p35; IL 12A; IL-12; IL12 p70; IL-12 p70; IL12A; IL-12A; IL12p70; IL12-p70; Interleukin 12 subunit alpha; NFSK; NKSF1; P35

免疫原

sf21, (I23-S328)+(R23-S219)

交叉反应

There is no detectable cross-reactivity with other relevant proteins.

检测范围

7.8 pg/ml - 500 pg/ml

基因名称

IL12A

基因名全称

interleukin 12A

Uniprot ID

O60595

文献引用格式

Human IL12(p70) ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0421)

背景资料

Interleukin(IL)-12 is a 70-KDa cytokine comprised of two disulfide-linked proteins(p35 and p40) and is essential for the initiation of effective immune response.[i] And the IL-12p70 is a heterodimer of p35 and p40 subunits; it is an important cytokine secreted by antigen-presenting cells in response to antigenic stimulation.[ii] Gene expression analysis of the IL-12 cytokine family subunits revealed that both strains induced high levels of p40(protein chain communal to IL-12 p70 and IL-23) as well as p19, a subunit of IL-23. Conversely only ACT- 18HS19 infection induced consistent transcription of IL-12 p35, a subunit of IL-12 p70.[iii] The standard product used in this kit is recombinant human IL-12 p70 with the molecular mass of 75 KDa.

显色时间

20-25分钟

试剂盒内容

Catalog number	Description	Quantity
EK0421-CAP	96-well plate precoated with anti- human IL-12(P70) antibody	1
EK0421-ST	Lyophilized recombinant human IL-12(P70) standard	10ng/tubex2
EK0421-DA	Biotinylated anti- human IL-12(P70) antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

批内差/批间差

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	18	51	294	19	55	310
Standard deviation	1.31	3.41	15.28	1.67	3.79	21.39
CV(%)	7.3	6.7	5.2	8.8	6.9	6.9

典型数据示例

Concentration(pg/ml)	0	7.8	15.6	31.3	62.5	125	250	500
O.D.	0.074	0.128	0.167	0.278	0.545	0.809	1.442	2.222

保存条件与有效期

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Human IL12(p70) ELISA Kit

EK0421

产品简介>

产品名称

规格/价格

指标别名

免疫原

交叉反应

检测范围

基因名称

基因名全称

Uniprot ID

文献引用格式

背景资料

显色时间

试剂盒内容

批内差/批间差

典型数据示例

保存条件与有效期

应用范围

检验物种

检测方法

样本类型

实验时长

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