Mouse IL12(p70) ELISA Kit

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EK0422

96T ￥2280
货期：现货

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产品名称

Mouse IL12(p70) ELISA Kit

规格/价格

96T/2280

指标别名

CLMF; CLMF p35; IL 12 subunit p35; IL 12A; IL-12; IL12 p70; IL-12 p70; IL12A; IL-12A; IL12p70; IL12-p70; Interleukin 12 subunit alpha; NFSK; NKSF1; P35

免疫原

Expression system for standard: NS0; Immunogen sequence: (M23-S335)+ (R23-A215)

交叉反应

There is no detectable cross-reactivity with other relevant proteins.

检测范围

15.6pg/ml-1000pg/ml

基因名称

IL12A

基因名全称

interleukin 12A

Uniprot ID

P43431

文献引用格式

Mouse IL12(p70) ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0422)

背景资料

Interleukin (IL)-12 is a 70-KDa cytokine comprised of two disulfide-linked proteins (p35 and p40) and is essential for the initiation of effective immune response. And the IL-12p70 is a heterodimer of p35 and p40 subunits; it is an important cytokine secreted by antigen-presenting cells in response to antigenic stimulation. Gene expression analysis of the IL-12 cytokine family subunits revealed that both strains induced high levels of p40 (protein chain communal to IL-12 p70 and IL-23) as well as p19, a subunit of IL-23. Conversely only ACT- 18HS19 infection induced consistent transcription of IL-12 p35, a subunit of IL-12 p70. The standard product used in this kit is recombinant mouse IL-12 p70 comprising of a 193 amino acids p35 chain and a 313 amino acids p40 chain connected by a disulfide bond.

试剂盒内容

Catalog number	Description	Quantity
EK0422-CAP	96-well plate precoated with anti- mouse IL-12(p70) antibody	1
EK0422-ST	Lyophilized recombinant mouse IL-12(p70) standard	10ng/tubex2
EK0422-DA	Biotinylated anti- mouse IL-12(p70) antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

批内差/批间差

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	37	150	410	33	147	442
Standard deviation	2.18	9.15	30.75	2.44	8.96	38.45
CV(%)	5.9%	6.1%	7.5%	7.4%	6.1%	8.7%

典型数据示例

Concentration(pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.044	0.088	0.116	0.194	0.338	0.618	1.144	2.010

保存条件与有效期

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Mouse IL12(p70) ELISA Kit

EK0422

产品简介>

产品名称

规格/价格

指标别名

免疫原

交叉反应

检测范围

基因名称

基因名全称

Uniprot ID

文献引用格式

背景资料

试剂盒内容

批内差/批间差

典型数据示例

保存条件与有效期

应用范围

检验物种

检测方法

样本类型

实验时长

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