Rat IL12(p70) ELISA Kit

说明书一键复制产品信息

EK1652

96T ￥2280
货期：现货

加入购物车立即购买

产品简介 其他介绍 引用文献 相关产品

产品简介>

产品名称

Rat IL12(p70) ELISA Kit

规格/价格

96T/2280

指标别名

CLMF; CLMF p35; IL 12 subunit p35; IL 12A; IL-12; IL12 p70; IL-12 p70; IL12A; IL-12A; IL12p70; IL12-p70; Interleukin 12 subunit alpha; NFSK; NKSF1; P35

免疫原

Expression system for standard: NS0; Immunogen sequence: (M23 - S335) + (R23 - S215)

交叉反应

There is no detectable cross-reactivity with other relevant proteins.

检测范围

15.6pg/ml-1000pg/ml

基因名称

Il12a

基因名全称

interleukin 12A

Uniprot ID

Q9R103

文献引用格式

Rat IL12(p70) ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1652)

背景资料

Interleukin (IL)-12 is a 70-KDa cytokine comprised of two disulfide-linked proteins (p35 and p40) and is essential for the initiation of effective immune response. And the IL-12p70 is a heterodimer of p35 and p40 subunits; it is an important cytokine secreted by antigen-presenting cells in response to antigenic stimulation. Gene expression analysis of the IL-12 cytokine family subunits revealed that both strains induced high levels of p40 (protein chain communal to IL-12 p70 and IL-23) as well as p19, a subunit of IL-23. Conversely only ACT- 18HS19 infection induced consistent transcription of IL-12 p35, a subunit of IL-12 p70. The standard product used in this kit is recombinant rat IL-12 p70 comprising of a 193 amino acids p35 chain and a 313 amino acids p40 chain connected by a disulfide bond.

显色时间

15-20分钟

试剂盒内容

Catalog number	Description	Quantity
EK1652-CAP	96-well plate precoated with anti- rat IL-12(p70) antibody	1
EK1652-ST	Lyophilized recombinant rat IL-12(p70) standard	10ng/tube×2
EK1652-DA	Biotinylated anti-rat IL-12(p70) antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

批内差/批间差

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	35	222	502	34	224	454
Standard deviation	1.92	15.76	27.61	2.07	19.93	29.05
CV(%)	5.5%	7.1%	5.5%	6.1%	8.9%	6.4%

典型数据示例

Concentration(pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.019	0.121	0.214	0.384	0.632	1.020	1.513	2.221

保存条件与有效期

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Rat IL12(p70) ELISA Kit

EK1652

产品简介>

产品名称

规格/价格

指标别名

免疫原

交叉反应

检测范围

基因名称

基因名全称

Uniprot ID

文献引用格式

背景资料

显色时间

试剂盒内容

批内差/批间差

典型数据示例

保存条件与有效期

应用范围

检验物种

检测方法

样本类型

其他介绍>

引用文献(0)>

清除

相关产品>

EK0421

EK0422

EZ0421

EZ0422

成功添加到购物车

产品中心

技术服务

技术资源

最新活动

关于BOSTER

联系订购

联系我们

关注我们

点击下列客服在线咨询：