产品名称
Human IL13 ELISA Kit
指标别名
ALRH; BHR1; IL 13; IL13; IL-13; interleukin 13; NC30; P600
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
15.6 pg/ml - 1000 pg/ml
文献引用格式
Human IL13 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0424)
背景资料
Interleukin 13 (IL-13) is a protein that in humans is encoded by the IL13 gene. IL-13 is cytokine secreted by many cell types, but especially T helper type 2 (Th2) cells which is an important mediator of allergic inflammation and disease. IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor (IL-4R alpha) and at least one of two known IL-13-specific binding chains. Most of the biological effects of IL-13, like those of IL-4, are linked to a single transcription factor, signal transducer and activator of transcription 6 (STAT6). The IL13 gene is clustered with IL3, IL5, IL4, and CSF2 on 5q.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0424-CAP | 96-well plate precoated with anti- human IL-13 antibody | 1 |
EK0424-ST | Lyophilized recombinant human IL-13 standard | 10ng/tube×2 |
EK0424-DA | Biotinylated anti- human IL-13 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 34 | 190 | 438 | 32 | 176 | 440 |
Standard deviation | 1.93 | 10.64 | 24.09 | 2.36 | 10.56 | 29.92 |
CV(%) | 5.7 | 5.6 | 5.5 | 7.4 | 6 | 6.8 |
典型数据示例
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.016 | 0.053 | 0.099 | 0.195 | 0.392 | 0.865 | 1.874 | 2.707 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)