产品名称
Mouse IL13 ELISA Kit
指标别名
ALRH; BHR1; IL 13; IL13; IL-13; interleukin 13; NC30; P600
免疫原
Expression system for standard: E.coli; Immunogen sequence: S26-F131
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Mouse IL13 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0425)
背景资料
Interleukin (IL)-13 is a major inducer of fibrosis in many chronic infectious and autoimmune diseases. Recombinant IL-13 protein inhibits inflammatory cytokine production induced by lipopolysaccharide in human peripheral blood monocytes. Moreover, it synergizes with IL-13 in regulating interferon-gamma synthesis in large granular lymphocytes. Interleukin-13 may be critical in regulating inflammatory and immune responses. IL-13 and IL-4 are two cytokines produced by T helper type 2 cells, mast cells, and basophils. In addition to their physiological roles, these cytokines are also implicated in pathological conditions such as asthma and allergy. The genes encoding human IL-4 and IL-13 are located on segment q23-31 of chromosome 5.4 Recombinant mouse IL-13 is a mixture of Ser26-Phe131 amino acids with the molecular mass of 11.5KDa.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0425-CAP | 96-well plate precoated with anti- mouse IL-13 antibody | 1 |
EK0425-ST | Lyophilized recombinant mouse IL-13 standard | 10ng/tubex2 |
EK0425-DA | Biotinylated anti- mouse IL-13 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 40 | 209 | 526 | 36 | 193 | 532 |
Standard deviation | 2.68 | 15.46 | 30.5 | 2.41 | 16.79 | 32.45 |
CV(%) | 6.7% | 7.4% | 5.8% | 6.7% | 8.7% | 6.1% |
典型数据示例
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.022 | 0.061 | 0.102 | 0.172 | 0.365 | 0.692 | 1.200 | 1.990 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)