指标别名
ALRH; BHR1; IL 13; IL13; IL-13; interleukin 13; NC30; P600
免疫原
Expression system for standard: E.coli; Immunogen sequence: T19-H131
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Rat IL13 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0900)
背景资料
Interleukin 13 (IL-13) is a protein that in humans is encoded by the IL13 gene. IL-13 is cytokine secreted by many cell types, but especially T helper type 2 (Th2) cells, that is an important mediator of allergic inflammation and disease. IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor (IL-4Ralpha) and at least one of two known IL-13-specific binding chains. Most of the biological effects of IL-13, like those of IL-4, are linked to a single transcription factor, signal transducer and activator of transcription 6 (STAT6). The IL13 gene is clustered with IL3, IL5, IL4, and CSF2 on 5q. Physical maps of the 5q23-q31 region show the following order: cen--IL3--CSF2--IL13--IL4--IL5--tel, with IL13 particularly close to IL4.
试剂盒内容
Catalog number | Description | Quantity |
EK0900-CAP | 96-well plate precoated with anti- rat IL-13 antibody | 1 |
EK0900-ST | Lyophilized recombinant rat IL-13 standard | 10ng/tube×2 |
EK0900-DA | Biotinylated anti- rat IL-13 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 37 | 170 | 412 | 36 | 164 | 394 |
Standard deviation | 2.73 | 10.37 | 22.66 | 3.09 | 12.95 | 27.58 |
CV(%) | 7.4% | 6.1% | 5.5% | 8.6% | 7.9% | 7% |
典型数据示例
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.156 | 0.186 | 0.237 | 0.326 | 0.512 | 0.866 | 1.434 | 2.107 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)