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Mouse Rantes/CCL5 ELISA Kit

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EK0495

  • 96T ¥2480
  • 货期: 现货

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产品名称
Mouse Rantes/CCL5 ELISA Kit
规格/价格
96T/2480
指标别名
C C motif chemokine 5; CCL5; chemokine (C C motif) ligand 5; D17S136E; EoCP; RANTES; SCYA5; SIS delta; SISd; Small inducible cytokine A5; T cell specific protein P228; T cell specific protein RANTES; TCP228
免疫原
Expression system for standard: E.coli; Immunogen sequence: S24-S91
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
15.6pg/ml-1000pg/ml
基因名称
CCL5
基因名全称
C-C motif chemokine 5
Uniprot ID
P30882  
文献引用格式
Mouse Rantes/CCL5 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0495)
背景资料
Rantes (regulated on activation normal T cell expressed and secreted) is one of the natural ligands for the chemokine receptor CCR5 and potently suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Peripheral blood mononuclear cells or CD4 (+) lymphocytes obtained from different individuals have wide variations in their ability to secrete RANTES. The Rantes gene product is predicted to be 10 kDa and, after cleavage of the signal peptide, approximately 8 kDa. Of the 68 residues, 4 are cysteines, and there are no sites for N-linked glycosylation. Rantes is expressed by cultured T cell lines that are Ag specific and growth factor dependent.
试剂盒内容
Catalog numberDescriptionQuantity
EK0495-CAP96-well plate precoated with anti- mouse Rantes antibody1
EK0495-STLyophilized recombinant mouse Rantes standard10ng/tubex2
EK0495-DABiotinylated anti- mouse Rantes antibody100ul(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex(ABC)100ul(dilution 1:100)
AR1106-1Sample diluent buffer30ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Intra-Assay Precision
Inter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)2919141626175442
Standard deviation1.658.9724.541.7110.8529.17
CV(%)5.7%4.7%5.9%6.6%6.2%6.6%
典型数据示例
Concentration(pg/ml)015.631.262.51252505001000
O.D.0.0230.0500.0780.1260.2520.5661.1902.186
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
mouse
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants Serum and Plasma (heparin, EDTA, citrate)
实验时长
3.5h

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