产品名称
Mouse Rantes/CCL5 Quick ELISA Kit
指标别名
C C motif chemokine 5; CCL5; chemokine (C C motif) ligand 5; D17S136E; EoCP; RANTES; SCYA5; SIS delta; SISd; Small inducible cytokine A5; T cell specific protein P228; T cell specific protein RANTES; TCP228
免疫原
Expression system for standard: E.coli; Immunogen sequence: S24-S91
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
chemokine (C-C motif) ligand 5
文献引用格式
Mouse Rantes/CCL5 Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK0495)
背景资料
Rantes(regulated on activation normal T cell expressed and secreted) is one of the natural ligands for the chemokine receptor CCR5 and potently suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Peripheral blood mononuclear cells or CD4(+) lymphocytes obtained from different individuals have wide variations in their ability to secrete RANTES. The Rantes gene product is predicted to be 10 kDa and, after cleavage of the signal peptide, approximately 8 kDa. Of the 68 residues, 4 are cysteines, and there are no sites for N-linked glycosylation. Rantes is expressed by cultured T cell lines that are Ag specific and growth factor dependent.
试剂盒内容
Catalog number | Description | Quantity |
FEK0495-CAP | Anti-Pre-coated 96-well Strip Microplate | 1 |
FEK0495-ST | Mouse Rantes standard | 10ng/tube×2 |
FEK0495-A | Mouse Rantes Antibody A | 3ml |
FEK0495-B | Mouse Rantes Antibody B | 3ml |
AR1106-1 | Sample diluent buffer | 15ml |
AR1106-7 | TBS-T Wash Buffer (25x) | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | Stop solution | 10ml |
PLA-SEA | Adhesive cover | 2 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 29 | 191 | 416 | 26 | 175 | 442 |
Standard deviation | 1.65 | 8.97 | 24.54 | 1.71 | 10.85 | 29.17 |
CV(%) | 5.7% | 4.7% | 5.9% | 6.6% | 6.2% | 6.6% |
典型数据示例
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.016 | 0.164 | 0.255 | 0.450 | 0.831 | 1.445 | 2.087 | 2.262 |
保存条件与有效期
Store at 4℃ for 6 months.
样本类型
Cell Culture Supernatants Serum and Plasma (heparin, EDTA, citrate)