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Rat Rantes/CCL5 ELISA Kit

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EK0496

  • 96T ¥2480
  • 货期: 现货

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产品名称
Rat Rantes/CCL5 ELISA Kit
规格/价格
96T/2480
指标别名
C C motif chemokine 5; CCL5; chemokine (C C motif) ligand 5; D17S136E; EoCP; RANTES; SCYA5; SIS delta; SISd; Small inducible cytokine A5; T cell specific protein P228; T cell specific protein RANTES; TCP228
免疫原
Expression system for standard: E.coli; Immunogen sequence: S24-S91
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
15.6pg/ml-1000pg/ml
基因名称
CCL5
基因名全称
chemokine (C-C motif) ligand 5
Uniprot ID
P50231  
文献引用格式
Rat Rantes/CCL5 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0496)
背景资料
Rantes (regulated on activation normal T cell expressed and secreted) is one of the natural ligands for the chemokine receptor CCR5 and potently suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Peripheral blood mononuclear cells or CD4 (+) lymphocytes obtained from different individuals have wide variations in their ability to secrete RANTES. The Rantes gene product is predicted to be 10 kDa and, after cleavage of the signal peptide, approximately 8 kDa. Of the 68 residues, 4 are cysteines, and there are no sites for N-linked glycosylation. Rantes is expressed by cultured T cell lines that are Ag specific and growth factor dependent.
显色时间
25-30分钟
试剂盒内容
Catalog numberDescriptionQuantity
EK0496-CAP96-well plate precoated with anti- rat Rantes antibody1
EK0496-STLyophilized recombinant rat Rantes standard10ng/tubex2
EK0496-DABiotinylated anti- rat Rantes antibody100ul(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex(ABC)100ul(dilution 1:100)
AR1106-1Sample diluent buffer30ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Sample123123
n161616242424
Mean(pg/ml)3420837437194349
Standard deviation2.2115.819.822.8114.9325.12
CV(%)6.5%7.6%5.3%7.6%7.7%7.2%
典型数据示例
Concentration(pg/ml)015.631.262.51252505001000
O.D.0.0780.1040.1240.1560.2950.5991.0181.889
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
rat
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)

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