产品名称
Rat Rantes/CCL5 ELISA Kit
指标别名
C C motif chemokine 5; CCL5; chemokine (C C motif) ligand 5; D17S136E; EoCP; RANTES; SCYA5; SIS delta; SISd; Small inducible cytokine A5; T cell specific protein P228; T cell specific protein RANTES; TCP228
免疫原
Expression system for standard: E.coli; Immunogen sequence: S24-S91
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
chemokine (C-C motif) ligand 5
文献引用格式
Rat Rantes/CCL5 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0496)
背景资料
Rantes (regulated on activation normal T cell expressed and secreted) is one of the natural ligands for the chemokine receptor CCR5 and potently suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Peripheral blood mononuclear cells or CD4 (+) lymphocytes obtained from different individuals have wide variations in their ability to secrete RANTES. The Rantes gene product is predicted to be 10 kDa and, after cleavage of the signal peptide, approximately 8 kDa. Of the 68 residues, 4 are cysteines, and there are no sites for N-linked glycosylation. Rantes is expressed by cultured T cell lines that are Ag specific and growth factor dependent.
试剂盒内容
Catalog number | Description | Quantity |
EK0496-CAP | 96-well plate precoated with anti- rat Rantes antibody | 1 |
EK0496-ST | Lyophilized recombinant rat Rantes standard | 10ng/tubex2 |
EK0496-DA | Biotinylated anti- rat Rantes antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 34 | 208 | 374 | 37 | 194 | 349 |
Standard deviation | 2.21 | 15.8 | 19.82 | 2.81 | 14.93 | 25.12 |
CV(%) | 6.5% | 7.6% | 5.3% | 7.6% | 7.7% | 7.2% |
典型数据示例
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.078 | 0.104 | 0.124 | 0.156 | 0.295 | 0.599 | 1.018 | 1.889 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)