产品名称
Mouse CD62L/SELL ELISA Kit
指标别名
CD62L; gp90 MEL; hLHRc; LAM 1; LAM1; LECAM1; Leu 8; Leukocyte adhesion molecule 1; LNHR; LSEL; L-selectin; Lyam 1; LYAM1; Lymph node homing receptor; PLNHR; selectin L; SELL; TQ1
免疫原
Expression system for standard: NS0; Immunogen sequence: W39-N332
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Mouse CD62L/SELL ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0504)
背景资料
L-selectin, also known as CD62L, is a cell adhesion molecule found on leukocytes. It belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups. It is cleaved by ADAM17.SELL (L-selectin)is a cell surface component that is a member of a family of adhesion/homing receptors which play important roles in leukocyte-endothelial cell interactions. The molecule is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins.1L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point2.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0504-CAP | 96-well plate precoated with anti- mouse sL-Selectin antibody | 1 |
EK0504-ST | Lyophilized recombinant mouse sL-Selectin standard | 10ng/tubex2 |
EK0504-DA | Biotinylated anti- mouse sL-Selectin antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 190 | 621 | 1771 | 204 | 669 | 1831 |
Standard deviation | 9.5 | 32.29 | 138.13 | 11.01 | 34.78 | 157.46 |
CV(%) | 5% | 5.2% | 7.8% | 5.4% | 5.2% | 8.6% |
典型数据示例
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.002 | 0.074 | 0.129 | 0.311 | 0.527 | 1.177 | 1.804 | 2.356 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)