产品名称
Rat CD62L/SELL ELISA Kit
指标别名
CD62L; gp90 MEL; hLHRc; LAM 1; LAM1; LECAM1; Leu 8; Leukocyte adhesion molecule 1; LNHR; LSEL; L-selectin; Lyam 1; LYAM1; Lymph node homing receptor; PLNHR; selectin L; SELL; TQ1
免疫原
Expression system for standard: NS0; Immunogen sequence: W39-N332
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Rat CD62L/SELL ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0937)
背景资料
L-selectin, also known as CD62L, is a cell adhesion molecule found on leukocytes. It belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups. It is cleaved by ADAM17.SELL (L-selectin) is a cell surface component that is a member of a family of adhesion/homing receptors which play important roles in leukocyte-endothelial cell interactions. The molecule is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins.1 L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point2.
试剂盒内容
Catalog number | Description | Quantity |
EK0937-CAP | 96-well plate precoated with anti- rat L-Selectin antibody | 1 |
EK0937-ST | Lyophilized recombinant rat L-Selectin standard | 40ng/tube×2 |
EK0937-DA | Biotinylated anti- rat L-Selectin antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 1474 | 3614 | 24600 | 1588 | 3716 | 23760 |
Standard deviation | 89.91 | 166.24 | 1279.2 | 100.04 | 178.36 | 1663.2 |
CV(%) | 6.1% | 4.6% | 5.2% | 6.3% | 4.8% | 7% |
典型数据示例
Concentration(pg/ml) | 0 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 | 40000 |
O.D. | 0.004 | 0.550 | 0.919 | 1.411 | 1.799 | 2.074 | 2.129 | 2.226 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)