产品名称
Human CD62L/SELL Quick ELISA Kit
指标别名
CD62L; gp90 MEL; hLHRc; LAM 1; LAM1; LECAM1; Leu 8; Leukocyte adhesion molecule 1; LNHR; LSEL; L-selectin; Lyam 1; LYAM1; Lymph node homing receptor; PLNHR; selectin L; SELL; TQ1
免疫原
Expression system for standard: NS0; Immunogen sequence: W39-N332
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Human CD62L/SELL Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK0503)
背景资料
L-selectin, also known as CD62L, is a cell adhesion molecule found on leukocytes. It belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups. It is cleaved by ADAM17.SELL(L-selectin)is a cell surface component that is a member of a family of adhesion/homing receptors which play important roles in leukocyte-endothelial cell interactions. The molecule is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins.1L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point2.
试剂盒内容
Catalog number | Description | Quantity |
FEK0503-CAP | Anti-Pre-coated 96-well Strip Microplate | 1 |
FEK0503-ST | Human L-Selectin standard | 10ng/tube×2 |
FEK0503-A | Human L-Selectin Antibody A | 3ml |
FEK0503-B | Human L-Selectin Antibody B | 3ml |
AR1106-1 | Sample diluent buffer | 15ml |
AR1106-7 | TBS-T Wash Buffer (25x) | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | Stop solution | 10ml |
PLA-SEA | Adhesive cover | 2 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 195 | 543 | 1239 | 187 | 595 | 1352 |
Standard deviation | 15.21 | 33.66 | 60.71 | 16.64 | 37.48 | 67.6 |
CV(%) | 7.8% | 6.2% | 4.9% | 8.9% | 6.3% | 5% |
典型数据示例
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.011 | 0.069 | 0.132 | 0.362 | 0.546 | 0.952 | 1.692 | 2.205 |
保存条件与有效期
Store at 4℃ for 6 months.
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)