产品名称
Human TIMP3 ELISA Kit
指标别名
HSMRK222; K222; K222TA2; Metalloproteinase inhibitor 3; Protein MIG 5; SFD; Timp 3; TIMP3; TIMP-3
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
156 pg/ml - 10000 pg/ml
基因名全称
TIMP metallopeptidase inhibitor 3
文献引用格式
Human TIMP3 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0523)
背景资料
The tissue inhibitors of metalloproteinases(TIMPs) are natural inhibitors of the matrix metalloproteinases, a group of zinc-binding endopeptidases involved in the degradation of the extracellular matrix. The TIMP3 gene is expressed in many tissues, with highest expression in the placenta. TIMP3 encodes a potent angiogenesis inhibitor and is mutated in Sorsby fundus dystrophy, a macular degenerative disease with submacular choroidal neovascularization. TIMP3 gene is mapped to 22q12.1-q13.2. Mutations in TIMP3 cause the autosomal dominant disorder Sorsby's fundus dystrophy(SFD).
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0523-CAP | 96-well plate precoated with anti- human TIMP-3 antibody | 1 |
EK0523-ST | Lyophilized recombinant human TIMP-3 standard | 10ng/tubex2 |
EK0523-DA | Biotinylated anti- human TIMP-3 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 233 | 1357 | 4383 | 248 | 1474 | 4049 |
Standard deviation | 16.31 | 92.27 | 271.74 | 21.08 | 120.86 | 327.96 |
CV(%) | 7 | 6.8 | 6.2 | 8.5 | 8.2 | 8.1 |
典型数据示例
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.008 | 0.052 | 0.079 | 0.160 | 0.356 | 0.826 | 1.693 | 2.485 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA) and Saliva