产品名称
Pig porcine TIMP3 ELISA Kit
指标别名
HSMRK222; K222; K222TA2; Metalloproteinase inhibitor 3; Protein MIG 5; SFD; Timp 3; TIMP3; TIMP-3
免疫原
Expression system for standard: NS0; Immunogen sequence: C24-P211
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Metalloproteinase inhibitor 3
文献引用格式
Pig porcine TIMP3 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0523-PO)
背景资料
The tissue inhibitors of metalloproteinases(TIMPs) are natural inhibitors of the matrix metalloproteinases, a group of zinc-binding endopeptidases involved in the degradation of the extracellular matrix. The TIMP3 gene is expressed in many tissues, with highest expression in the placenta. TIMP3 encodes a potent angiogenesis inhibitor and is mutated in Sorsby fundus dystrophy, a macular degenerative disease with submacular choroidal neovascularization. TIMP3 gene is mapped to 22q12.1-q13.2. Mutations in TIMP3 cause the autosomal dominant disorder Sorsby's fundus dystrophy(SFD).
试剂盒内容
Catalog number | Description | Quantity |
EK0523-PO-CAP | Anti-Pig TIMP3 Pre-coated 96-well strip microplate | 1 |
EK0523-PO-ST | Pig TIMP3 Standard | 10ng/tube×2 |
EK0523-PO-DA | Pig TIMP3 Biotinylated antibody (100x) | 100ul |
AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
AR1106-1 | Sample Diluent | 30ml |
AR1106-2 | Antibody Diluent | 12ml |
AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop solution | 10ml |
AR1106-5 | Wash Buffer (25x) | 20ml |
PLA-SEA | Adhesive plate sealers | 4 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 1250 | 2687 | 5353 | 1430 | 2832 | 5657 |
Standard deviation | 72.5 | 110.2 | 192.7 | 95.81 | 147.3 | 277.2 |
CV (%) | 5.8% | 4.1% | 3.6% | 6.7% | 5.2% | 4.9% |
典型数据示例
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.019 | 0.080 | 0.118 | 0.219 | 0.437 | 0.808 | 1.571 | 2.420 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA) and Saliva