Human BAFF/TNFSF13B ELISA Kit

96T/2480

TNF superfamily member 13b; BAFF; BLYS; CD257; DTL; TALL-1; TALL1; THANK; TNFSF20; TNLG7A; ZTNF4; TNFSF13B

NSO, A134-L285

There is no detectable cross-reactivity with other relevant proteins.

62.5 pg/ml - 4000 pg/ml

TNFSF13B

tumor necrosis factor (ligand) superfamily, member 13b

Q9Y275  

Human BAFF/TNFSF13B ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0663)

BAFF was regularly detected by enzyme-linked immunosorbent assay in brain tissue lysates and in normal spinal fluid, and in astrocytes by double fluorescence microscopy. BAFF was localized in astrocytes close to BAFF-R-expressing immune cells. BAFF receptors were strongly expressed in situ in primary central nervous system(CNS) lymphomas.1 The TNF superfamily member B cell-activating factor(BAFF) plays an important role in humoral immunity and in autoimmune diseases, including RA.Local BAFF gene targeting inhibited proinflammatory cytokine expression, suppressed generation of plasma cells and Th17 cells, and markedly ameliorated joint pathology.2 The B cell activating factor BAFF(BlyS/TALL-1/zTNF4) is a tumor necrosis factor(TNF)-related ligand that promotes B cell survival and binds to three receptors(BCMA, TACI, and the recently described BAFF-R).3 Human BAFF was mapped to chromosome 13q32-34.4 The standard used in this kit is recombinant soluble human BAFF(A134-L295) with the molecular mass of 19.6KDa.

15-20分钟

Catalog number	Description	Quantity
EK0663-CAP	96-well plate precoated with anti- human BAFF antibody	1
EK0663-ST	Lyophilized recombinant human BAFF standard	10ng/tubex2
EK0663-DA	Biotinylated anti- human BAFF antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	127	522	1229	124	548	1268
Standard deviation	5.46	38.62	79.88	5.7	47.67	103.97
CV(%)	4.3	7.4	6.5	4.6	8.7	8.2

Concentration(pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.008	0.075	0.144	0.252	0.530	0.999	1.818	2.551

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

酶联免疫双抗夹心法

Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)

3.5h