Human BAFF/TNFSF13B Quick ELISA Kit

96T/3580

TNF superfamily member 13b; BAFF; BLYS; CD257; DTL; TALL-1; TALL1; THANK; TNFSF20; TNLG7A; ZTNF4; TNFSF13B

Expression system for standard: NS0; Immunogen sequence: A134-L285

There is no detectable cross-reactivity with other relevant proteins.

62.5pg/ml-4000pg/ml

TNFSF13B

tumor necrosis factor (ligand) superfamily, member 13b

Q9Y275  

Human BAFF/TNFSF13B Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK0663)

BAFF was regularly detected by enzyme-linked immunosorbent assay in brain tissue lysates and in normal spinal fluid, and in astrocytes by double fluorescence microscopy. BAFF was localized in astrocytes close to BAFF-R-expressing immune cells. BAFF receptors were strongly expressed in situ in primary central nervous system(CNS) lymphomas.1 The TNF superfamily member B cell-activating factor(BAFF) plays an important role in humoral immunity and in autoimmune diseases, including RA.Local BAFF gene targeting inhibited proinflammatory cytokine expression, suppressed generation of plasma cells and Th17 cells, and markedly ameliorated joint pathology.2 The B cell activating factor BAFF(BlyS/TALL-1/zTNF4) is a tumor necrosis factor(TNF)-related ligand that promotes B cell survival and binds to three receptors(BCMA, TACI, and the recently described BAFF-R).3 Human BAFF was mapped to chromosome 13q32-34.4 The standard used in this kit is recombinant soluble human BAFF(A134-L295) with the molecular mass of 19.6KDa.

15分钟

Catalog number	Description	Quantity
FEK0663-CAP	96-well plate precoated with anti-Human TNFSF13B antibody	1
FEK0663-ST	lyophilized recombinant Human TNFSF13B standard	10ng/tube×2
FEK0663-A	Human TNFSF13B Antibody A	3ml
FEK0663-B	Human TNFSF13B Antibody B	3ml
AR1106-1	Sample Diluent	15ml
AR1106-7	TBS-T Wash Buffer (25x)	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
PLA-SEA	Adhesive Plate Sealers	2

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	125	539	1336	125	530	1452
Standard deviation	6.12	40.42	78.82	6.00	43.99	110.35
CV(%)	4.9%	7.5%	5.9%	4.8%	8.3%	7.6%

Concentration(pg/ml)	0	62.5	125	250	500	1,000	2,000	4,000
O.D.	0.018	0.088	0.119	0.223	0.391	0.716	1.262	1.941

Store at 4℃ for 6 months.

ELISA

human

酶联免疫双抗夹心法

Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)