产品名称
Mouse BAFF/TNFSF13B ELISA Kit
指标别名
TNF superfamily member 13b; BAFF; BLYS; CD257; DTL; TALL-1; TALL1; THANK; TNFSF20; TNLG7A; ZTNF4; TNFSF13B
免疫原
Expression system for standard: NS0; Immunogen sequence: A127-L309
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
tumor necrosis factor (ligand) superfamily, member 13b
文献引用格式
Mouse BAFF/TNFSF13B ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0664)
背景资料
B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFLSF13B gene.1,2 BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells.3All these ligands act as heterotrimers (i.e. three of the same molecule) interacting with heterotrimeric receptors4, although BAFF has been known to be active as either a hetero- or homotrimer.5 The standard used in this kit is recombinant mouse soluble BAFF (A127-L309) with molecular weight 23.2KDa.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0664-CAP | 96-well plate precoated with anti- mouse BAFF antibody | 1 |
| EK0664-ST | Lyophilized recombinant mouse BAFF standard | 10ng/tubex2 |
| EK0664-DA | Biotinylated anti- mouse BAFF antibody | 100ul(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 82 | 293 | 1221 | 76 | 287 | 1209 |
| Standard deviation | 6.47 | 15.52 | 67.15 | 7.14 | 16.07 | 72.54 |
| CV(%) | 7.9% | 5.3% | 5.5% | 9.4% | 5.6% | 6% |
典型数据示例
| Concentration(pg/ml) | 0 | 46.9 | 94 | 188 | 375 | 750 | 1500 | 3000 |
| O.D. | 0.078 | 0.147 | 0.201 | 0.315 | 0.507 | 0.891 | 1.516 | 2.346 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)
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