产品名称
Human IP10/CXCL10 ELISA Kit
指标别名
C X C motif chemokine 10; C7; crg 2; CXCL10; CXCL10/IP10; Gamma IP10; gIP 10; IFI10; INP10; IP 10; IP10; mob 1; SCYB10; Small inducible cytokine B10
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
31.2 pg/ml - 2000 pg/ml
基因名全称
chemokine (C-X-C motif) ligand 10
文献引用格式
Human IP10/CXCL10 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0735)
背景资料
Chemokine(C-X-C motif) ligand 10(CXCL10) or IP-10 is a small cytokine belonging to the CXC chemokine family that is also known as 10 kDa interferon-gamma-induced protein(gamma-IP10 or IP-10). CXCL10 is secreted by several cell types in response to IFN-gamma. These cell types include monocytes, endothelial cells and fibroblasts. CXCL10 has been attributed to several roles, such as chemoattraction for monocytes/macrophages, T cells, NK cells, and dendritic cells, promotion of T cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation and angiogenesis.It is a potent inhibitor of angiogenesis in vivo. The gene for CXCL10 is located on human chromosome 4 in a cluster among several other CXC chemokines. It is a RAS target gene and is overexpressed in the majority of colorectal cancers.This chemokine elicits its effects by binding to the cell surface chemokine receptor CXCR3.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0735-CAP | 96-well plate precoated with anti- human CXCL10 antibody | 1 |
EK0735-ST | Lyophilized recombinant human CXCL10 standard | 10ng/tube×2 |
EK0735-DA | Biotinylated anti- human CXCL10 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 60 | 243 | 879 | 64 | 223 | 865 |
Standard deviation | 3.24 | 19.19 | 45.7 | 4.28 | 19.4 | 47.57 |
CV(%) | 5.4 | 7.9 | 5.2 | 6.7 | 8.7 | 5.5 |
典型数据示例
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.011 | 0.044 | 0.088 | 0.151 | 0.279 | 0.649 | 1.271 | 2.004 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA), Saliva and Urine