产品名称
Mouse IP10/CXCL10 ELISA Kit
指标别名
C X C motif chemokine 10; C7; crg 2; CXCL10; CXCL10/IP10; Gamma IP10; gIP 10; IFI10; INP10; IP 10; IP10; mob 1; SCYB10; Small inducible cytokine B10
免疫原
Expression system for standard: E.coli; Immunogen sequence: I22-P98
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
C-X-C motif chemokine 10
文献引用格式
Mouse IP10/CXCL10 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0736)
背景资料
Chemokine (C-X-C motif) ligand 10 (CXCL10) or IP-10 is a small cytokine belonging to the CXC chemokine family that is also known as 10 kDa interferon-gamma-induced protein (gamma-IP10 or IP-10). CXCL10 is secreted by several cell types in response to IFN-gamma. These cell types include monocytes, endothelial cells and fibroblasts. CXCL10 has been attributed to several roles, such as chemoattraction for monocytes/macrophages, T cells, NK cells, and dendritic cells, promotion of T cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation and angiogenesis.It is a potent inhibitor of angiogenesis in vivo. The gene for CXCL10 is located on human chromosome 4 in a cluster among several other CXC chemokines. It is a RAS target gene and is overexpressed in the majority of colorectal cancers.This chemokine elicits its effects by binding to the cell surface chemokine receptor CXCR3.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0736-CAP | 96-well plate precoated with anti- mouse CXCL10 antibody | 1 |
| EK0736-ST | Lyophilized recombinant mouse CXCL10 standard | 10ng/tube×2 |
| EK0736-DA | Biotinylated anti- mouse CXCL10 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 105 | 245 | 923 | 110 | 248 | 879 |
| Standard deviation | 7.87 | 13.72 | 47.99 | 10.23 | 18.6 | 56.25 |
| CV(%) | 7.5% | 5.6% | 5.2% | 9.3% | 7.5% | 6.4% |
典型数据示例
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.024 | 0.062 | 0.088 | 0.163 | 0.286 | 0.516 | 1.002 | 1.854 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin)
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