产品名称
Human Galectin-1/LGALS1 ELISA Kit
指标别名
14 kDa laminin binding protein; 14 kDa lectin; Gal 1; GAL1; Galaptin; Galectin 1; Galectin-1; HBL; HLBP14; HPL; Lactose binding lectin 1; LGALS1; S Lac lectin 1
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
156 pg/ml - 10000 pg/ml
基因名全称
lectin, galactoside-binding, soluble, 1
文献引用格式
Human Galectin-1/LGALS1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0762)
背景资料
Galectin-1 is a protein that in humans is encoded by the LGALS1 gene. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-1 may act as an autocrine negative growth factor that regulates cell proliferation. Its expression in Hodgkin Lymphoma has also been shown to mediate immunosuppression of CD8+ T-cells. Additionally, Galectin-1 is thought to play a role in creating immune tolerance in pregnancy.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0762-CAP | 96-well plate precoated with anti- human Galectin-1 antibody | 1 |
EK0762-ST | Lyophilized recombinant human Galectin-1 standard | 10ng/tube×2 |
EK0762-DA | Biotinylated anti- human Galectin-1 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 232 | 1786 | 5647 | 229 | 1822 | 5807 |
Standard deviation | 13.45 | 121.44 | 355.76 | 13.96 | 147.58 | 476.17 |
CV(%) | 5.8 | 6.8 | 6.3 | 6.1 | 8.1 | 8.2 |
典型数据示例
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.008 | 0.077 | 0.156 | 0.295 | 0.526 | 0.962 | 1.564 | 2.104 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)