产品名称
Rat Galectin-1/LGALS1 ELISA Kit
指标别名
14 kDa laminin binding protein; 14 kDa lectin; Gal 1; GAL1; Galaptin; Galectin 1; Galectin-1; HBL; HLBP14; HPL; Lactose binding lectin 1; LGALS1; S Lac lectin 1
免疫原
Expression system for standard: E.coli; Immunogen sequence: A2-E135
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Rat Galectin-1/LGALS1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1589)
背景资料
Galectin-1 is a protein that in humans is encoded by the LGALS1 gene. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-1 may act as an autocrine negative growth factor that regulates cell proliferation. Its expression in Hodgkin Lymphoma has also been shown to mediate immunosuppression of CD8+ T-cells. Additionally, Galectin-1 is thought to play a role in creating immune tolerance in pregnancy.
试剂盒内容
Catalog number | Description | Quantity |
EK1588-CAP | 96-well plate precoated with anti- rat Galectin-1 antibody | 1 |
EK1588-ST | Lyophilized recombinant rat Galectin-1 standard | 10ng/tubex2 |
EK1588-DA | Biotinylated anti- rat Galectin-1 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 243 | 1504 | 5437 | 262 | 1512 | 5561 |
Standard deviation | 15.31 | 85.71 | 260.98 | 17.29 | 110.4 | 428.2 |
CV(%) | 6.3% | 5.7% | 4.8% | 6.6% | 7.3% | 7.7% |
典型数据示例
Concentration(pg/ml) | 0 | 156 | 313 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.079 | 0.174 | 0.258 | 0.407 | 0.684 | 1.056 | 1.570 | 2.274 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)