产品名称
Mouse Galectin-1/LGALS1 ELISA Kit
指标别名
14 kDa laminin binding protein; 14 kDa lectin; Gal 1; GAL1; Galaptin; Galectin 1; Galectin-1; HBL; HLBP14; HPL; Lactose binding lectin 1; LGALS1; S Lac lectin 1
免疫原
Expression system for standard: E.coli; Immunogen sequence: A2-E135
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Mouse Galectin-1/LGALS1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0763)
背景资料
Galectin-1 is a protein that in humans is encoded by the LGALS1 gene.The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. LGALS1 may act as an autocrine negative growth factor that regulates cell proliferation.Baldini et al. stated that the mouse beta-galactoside-binding protein is an autocrine regulator of cell proliferation with a role in the maintenance of G0 and in the control of G2 traverse. They found that galectin-1 was expressed in a subset of slowly dividing subventricular zone astrocytes, which included the neural stem cells. Intraventricular infusion experiments and phenotypic analysis of knockout mice showed that galectin-1 was an endogenous factor that promoted the proliferation of neural stem cells in adult mouse brain. The standard product used in this kit is recombinant mouse GALECTIN-1, Ala2-Glu135, with the molecular mass of 15KDa.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0763-CAP | 96-well plate precoated with anti- mouse Galectin-1 antibody | 1 |
EK0763-ST | Lyophilized recombinant mouse Galectin-1 standard | 10ng/tube×2 |
EK0763-DA | Biotinylated anti- mouse Galectin-1 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 343 | 1747 | 4774 | 318 | 1697 | 4684 |
Standard deviation | 22.29 | 132.77 | 300.76 | 20.67 | 144.24 | 304.46 |
CV(%) | 6.5% | 7.6% | 6.3% | 6.5% | 8.5% | 6.5% |
典型数据示例
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.151 | 0.224 | 0.303 | 0.487 | 0.722 | 1.082 | 1.811 | 2.344 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)