产品名称
Human PCSK9 ELISA Kit
指标别名
FH3; HCHOLA3; LDLCQ1; NARC 1; NARC1; PC9; PCSK9; Proprotein convertase 9
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
156 pg/ml - 10000 pg/ml
基因名全称
proprotein convertase subtilisin/kexin type 9
文献引用格式
Human PCSK9 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1147)
背景资料
Proprotein convertase subtilisin/kexin type 9, also known as PCSK9, is an enzyme that in humans is encoded by the PCSK9 gene. This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. By genomic sequence analysis, the PCSK9 gene was mapped to chromosome 1p32. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A(EGF-A) domain of the low-density lipoprotein receptor(LDLR), inducing LDLR degradation. Reduced LDLR levels result in decreased metabolism of low-density lipoproteins(LDL), which could lead to hypercholesterolemia. PCSK9 may also have a role in the differentiation of cortical neurons.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK1147-CAP | 96-well plate precoated with anti- human PCSK9 antibody | 1 |
| EK1147-ST | Lyophilized recombinant human PCSK9 standard | 10ng/tube×2 |
| EK1147-DA | Biotinylated anti- human PCSK9 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 229 | 1991 | 5404 | 215 | 1927 | 5944 |
| Standard deviation | 16.03 | 125.43 | 243.18 | 17.63 | 132.96 | 285.31 |
| CV(%) | 7 | 6.3 | 4.5 | 8.2 | 6.9 | 4.8 |
典型数据示例
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.056 | 0.121 | 0.172 | 0.273 | 0.456 | 0.808 | 1.409 | 2.289 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)