产品名称
Mouse PCSK9 ELISA Kit
指标别名
FH3; HCHOLA3; LDLCQ1; NARC 1; NARC1; PC9; PCSK9; Proprotein convertase 9
免疫原
Expression system for standard: NS0; Immunogen sequence: S156-Q694
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Proprotein convertase subtilisin/kexin type 9
文献引用格式
Mouse PCSK9 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1148)
背景资料
Proprotein convertase subtilisin/kexin type 9, also known as PCSK9, is an enzyme that in humans is encoded by the PCSK9 gene. This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. By genomic sequence analysis, the PCSK9 gene was mapped to chromosome 1p32. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. Reduced LDLR levels result in decreased metabolism of low-density lipoproteins (LDL), which could lead to hypercholesterolemia. PCSK9 may also have a role in the differentiation of cortical neurons.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK1148-CAP | 96-well plate precoated with anti- mouse PCSK9 antibody | 1 |
| EK1148-ST | Lyophilized recombinant mouse PCSK9 standard | 10ng/tube×2 |
| EK1148-DA | Biotinylated anti- mouse PCSK9 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 95 | 662 | 1007 | 93 | 661 | 1006 |
| Standard deviation | 3.99 | 48.32 | 78.54 | 4.92 | 48.25 | 96.57 |
| CV(%) | 4.2% | 7.3% | 7.8% | 5.3% | 7.3% | 9.6% |
典型数据示例
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.006 | 0.092 | 0.158 | 0.273 | 0.508 | 0.907 | 1.647 | 2.24 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)