指标别名
FH3; HCHOLA3; LDLCQ1; NARC 1; NARC1; PC9; PCSK9; Proprotein convertase 9
免疫原
Expression system for standard: NS0; Immunogen sequence: S156-Q694
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
proprotein convertase subtilisin/kexin type 9
文献引用格式
Rat PCSK9 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1701)
背景资料
PCSK9 is the 9th member of the proprotein convertase family of proteins that activate other proteins.PCSK9 has medical importance because it acts in lipoprotein homeostasis. PCSK9 is ubiquitously expressed in many tissues and cell types. PCSK9 binds to the receptor for low-density lipoprotein particles (LDL), which typically transport 3,000 to 6,000 fat molecules (including cholesterol) per particle, within extracellular fluid. The LDL receptor (LDLR), on liver and other cell membranes, binds and initiates ingestion of LDL-particles from extracellular fluid into cells, thus reducing LDL particle concentrations.
试剂盒内容
Catalog number | Description | Quantity |
EK1701-CAP | 96-well plate precoated with anti- rat PCSK9 antibody | 1 |
EK1701-ST | Lyophilized recombinant rat PCSK9 standard | 10ng/tubex2 |
EK1701-DA | Biotinylated anti- rat PCSK9 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 140 | 555 | 1161 | 145 | 563 | 1332 |
Standard deviation | 6.58 | 30.53 | 68.5 | 9.28 | 37.72 | 99.9 |
CV(%) | 4.7% | 5.5% | 5.9% | 6.4% | 6.7% | 7.5% |
典型数据示例
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.061 | 0.122 | 0.175 | 0.276 | 0.520 | 0.952 | 1.408 | 2.428 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)