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Human VE-Cadherin/CDH5 ELISA Kit

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EK1317

  • 96T ¥2480
  • 货期: 现货

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产品名称
Human VE-Cadherin/CDH5 ELISA Kit
规格/价格
96T/2480
指标别名
7B4; 7B4 antigen; Cadherin 5; CD144; CDH5; FLJ17376; Vascular endothelial cadherin; VE cadherin
免疫原
NSO, D48-Q599
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
312 pg/ml - 20000 pg/ml
基因名称
CDH5
基因名全称
cadherin 5, type 2 (vascular endothelium)
Uniprot ID
P33151  
文献引用格式
Human VE-Cadherin/CDH5 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1317)
背景资料
Cadherin 5, type 2 or VE-cadherin also known as CD144, is encoded by the gene CDH5. It is a classical cadherin from the cadherin superfamily. This gene is mapped to mouse chromosome 8. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It has found that PECAM1, VE-cadherin and VEGFR2 together are sufficient to confer responsiveness to flow in heterologous cells.
显色时间
15-20分钟
试剂盒内容
Catalog numberDescriptionQuantity
EK1317-CAP96-well plate precoated with anti- human VE-Cadherin antibody1
EK1317-STLyophilized recombinant human VE-Cadherin standard20ng/tube×2
EK1317-DABiotinylated anti- human VE-Cadherin antibody100μl(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex (ABC)100μl(dilution 1:100)
AR1106-1Sample diluent buffer30ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Intra-Assay Precision
Inter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)6701989753470320696886
Standard deviation40.87155.14587.6549.21190.34667.94
CV(%)6.17.87.879.29.7
典型数据示例
Concentration(pg/ml)03126251250250050001000020000
O.D.0.0960.2200.2860.5050.8141.2791.9702.189
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
human
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)
实验时长
3.5h

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