产品名称
Mouse VE-Cadherin/CDH5 ELISA Kit
指标别名
7B4; 7B4 antigen; Cadherin 5; CD144; CDH5; FLJ17376; Vascular endothelial cadherin; VE cadherin
免疫原
Expression system for standard: NS0; Immunogen sequence: D46-Q599
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Mouse VE-Cadherin/CDH5 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1318)
背景资料
Cadherin 5, type 2 or VE-cadherin also known as CD144, is encoded by the gene CDH5. It is a classical cadherin from the cadherin superfamily. This gene is mapped to mouse chromosome 8. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It has found that PECAM1, VE-cadherin and VEGFR2 together are sufficient to confer responsiveness to flow in heterologous cells.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1318-CAP | 96-well plate precoated with anti- mouse VE-Cadherin antibody | 1 |
EK1318-ST | Lyophilized recombinant mouse VE-Cadherin standard | 20ng/tube×2 |
EK1318-DA | Biotinylated anti- mouse VE-Cadherin antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 850 | 2556 | 9850 | 867 | 2722 | 9885 |
Standard deviation | 45.9 | 161.02 | 502.35 | 62.42 | 206.87 | 533.79 |
CV(%) | 5.4% | 6.3% | 5.1% | 7.2% | 7.6% | 5.4% |
典型数据示例
Concentration(pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
O.D. | 0.045 | 0.133 | 0.194 | 0.310 | 0.583 | 0.986 | 1.785 | 2.576 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)