产品名称
Rat VE-Cadherin/CDH5 ELISA Kit
指标别名
7B4; 7B4 antigen; Cadherin 5; CD144; CDH5; FLJ17376; Vascular endothelial cadherin; VE cadherin
免疫原
Expression system for standard: NS0; Immunogen sequence: D46 - Q599
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Rat VE-Cadherin/CDH5 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1916)
背景资料
Cadherin 5, type 2 or VE-cadherin also known as CD144, is a type of cadherin. It is encoded by the human gene CDH5. This gene is mapped to 19p14. VE-cadherin is a classical cadherin from the cadherin superfamily and the gene is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. The encoded protein is a calcium-dependent cell–cell adhesion glycoprotein composed of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions.
试剂盒内容
Catalog number | Description | Quantity |
EK1916-CAP | 96-well plate precoated with anti- rat VE-Cadherin/CD144 antibody | 1 |
EK1916-ST | Lyophilized recombinant rat VE-Cadherin/CD144 standard | 20ng/tube×2 |
EK1916-DA | Biotinylated anti- rat VE-Cadherin/CD144 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 399 | 1935 | 8758 | 443 | 2194 | 8947 |
Standard deviation | 21.95 | 123.84 | 507.96 | 27.47 | 112.11 | 653.13 |
CV(%) | 5.5% | 6.4% | 5.8% | 6.2% | 5.1% | 7.3% |
典型数据示例
Concentration(pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
O.D. | 0.012 | 0.147 | 0.264 | 0.463 | 0.819 | 1.613 | 2.165 | 2.793 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)