产品名称
Human APOE ELISA Kit
指标别名
AD2; Apo E; APOE; apoe4; apolipoprotein E; LPG
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
3.12 ng/ml - 200 ng/ml
文献引用格式
Human APOE ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1455)
背景资料
Apolipoprotein E (APOE) is a class of apolipoprotein found in the chylomicron and Intermediate-density lipoprotein (IDLs) that is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. In peripheral tissues, APOE is primarily produced by the liver and macrophages, and mediates cholesterol metabolism in an isoform- dependent manner. In the central nervous system, APOE is mainly produced by astrocytes, and transports cholesterol to neurons via APOE receptors, which are members of the low density lipoprotein receptor gene family. This protein is involved in Alzheimer’s disease and cardiovascular disease.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1455-CAP | 96-well plate precoated with anti- human APOE antibody | 1 |
EK1455-ST0 | Lyophilized recombinant human APOE standard | 200ng/tube×2 |
EK1455-DA | Biotinylated anti- human APOE antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 7334 | 30860 | 101114 | 7480 | 33927 | 97046 |
Standard deviation | 506.04 | 2191.06 | 7886.89 | 598.4 | 2917.72 | 7957.77 |
CV(%) | 6.9 | 7.1 | 7.8 | 8 | 8.6 | 8.2 |
典型数据示例
Concentration(ng/ml) | 0 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100 | 200 |
O.D. | 0.010 | 0.073 | 0.138 | 0.243 | 0.484 | 0.907 | 1.475 | 2.204 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA), Saliva, milk and Urine