产品名称
Human APOE EZ-Set™ ELISA Kit (DIY Antibody Pairs)
指标别名
AD2; Apo E; APOE; apoe4; apolipoprotein E; LPG
免疫原
Expression system for standard: E.coli; Immunogen sequence: K19-H317
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
3.12 ng/ml - 200 ng/ml
文献引用格式
Human APOE EZ-Set™ ELISA Kit (DIY Antibody Pairs) (Boster Biological Technology, Wuhan, China. Catalog#EZ1455)
背景资料
Apolipoprotein E (APOE) is a class of apolipoprotein found in the chylomicron and Intermediate-absorbance lipoprotein (IDLs) that is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. In peripheral tissues, APOE is primarily produced by the liver and macrophages, and mediates cholesterol metabolism in an isoform- dependent manner. In the central nervous system, APOE is mainly produced by astrocytes, and transports cholesterol to neurons via APOE receptors, which are members of the low absorbance lipoprotein receptor gene family. This protein is involved in Alzheimer’s disease and cardiovascular disease.
试剂盒内容
| Catalog number |
Description |
Quantity |
Storage of opened/reconstituted material |
| EZ1455-CA |
Rabbit anti- human APOE polyclonal antibody (Capture Antibody) |
500 µl, 0.4 mg/mL
(recommended dilution 1:100) |
Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit.
|
| EZ1455-DA |
Biotinylated goat anti- human APOE polyclonal antibody (Detection Antibody) |
500 µl, 2 µg/mL
(recommended dilution 1:100) |
| AR1103 |
Avidin-Biotin-Peroxidase Complex (ABC) |
500 µl (recommended dilution 1:100) |
| EZ1455-ST |
Lyophilized recombinant human APOE standard |
200 ng/tube×3
|
Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the
expiration date of the kit. |
典型数据示例
| Concentration (ng/ml) | 0 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100 | 200 |
| O.D. | 0.01 | 0.073 | 0.138 | 0.243 | 0.484 | 0.907 | 1.475 | 2.004 |
保存条件与有效期
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
需要自备的试剂
1. Microplate reader in standard size.
2. Automated plate washer.
3. Incubator.
4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
5. Clean tubes and Eppendorf tubes.
6. 96 well microplate (Cat# AR1100)
7. Plate Sealers.
8. Capture Antibody Diluent: PBS.
9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
10. Substrate Solution:
Tetramethylbenzidine (Cat# AR1104)
11. Stop Solution: 2 N H2SO4 (Cat# AR1105)
12.Wash Buffer (PBS and PBS-T). Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
*Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)
*样品准备的相关试剂也不含在试剂盒中。
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA), Saliva, milk and Urine
联系我们
关注我们
鄂公网安备 42018502007312号 
积分商城 
购物车 
登录/注册 
您当前的位置： 
说明书
成功添加到购物车
微信客服
微信扫一扫立即咨询 