产品名称
Human APOE Quick ELISA Kit
指标别名
AD2; Apo E; APOE; apoe4; apolipoprotein E; LPG
免疫原
Expression system for standard: E.coli; Immunogen sequence: K19-H317
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Human APOE Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK1455)
背景资料
Apolipoprotein E (APOE) is a class of apolipoprotein found in the chylomicron and Intermediate-absorbance lipoprotein (IDLs) that is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. In peripheral tissues, APOE is primarily produced by the liver and macrophages, and mediates cholesterol metabolism in an isoform- dependent manner. In the central nervous system, APOE is mainly produced by astrocytes, and transports cholesterol to neurons via APOE receptors, which are members of the low absorbance lipoprotein receptor gene family. This protein is involved in Alzheimer’s disease and cardiovascular disease.
试剂盒内容
Catalog number | Description | Quantity |
FEK0663-CAP | 96-well plate precoated with anti-Human APOE antibody | 1 |
FEK0663-ST | lyophilized recombinant Human APOE standard | 10ng/tube×2 |
FEK0663-A | Human APOE Antibody A | 3ml |
FEK1455-B | Human APOE Antibody B | 3ml |
AR1106-1 | Sample Diluent | 15ml |
AR1106-7 | TBS-T Wash Buffer (25x) | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop Solution | 10ml |
PLA-SEA | Adhesive Plate Sealers | 2 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 7336 | 30862 | 101167 | 7284 | 33941 | 97075 |
Standard deviation | 454.83 | 2129.47 | 7587.52 | 590.00 | 2884.98 | 8542.60 |
CV(%) | 6.2% | 6.9% | 7.5% | 8.1% | 8.5% | 8.8% |
典型数据示例
Concentration(ng/ml) | 0 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100 | 200 |
O.D. | 0.003 | 0.143 | 0.256 | 0.448 | 0.731 | 1.041 | 1.413 | 1.650 |
保存条件与有效期
Store at 4℃ for 6 months.
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA), Saliva, milk and Urine