Human APOE Quick ELISA Kit

96T/3580

AD2; Apo E; APOE; apoe4; apolipoprotein E; LPG

Expression system for standard: E.coli; Immunogen sequence: K19-H317

There is no detectable cross-reactivity with other relevant proteins.

<50pg/ml

3.12ng/ml-200ng/ml

APOE

apolipoprotein E

P02649

Human APOE Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK1455)

Apolipoprotein E (APOE) is a class of apolipoprotein found in the chylomicron and Intermediate-absorbance lipoprotein (IDLs) that is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. In peripheral tissues, APOE is primarily produced by the liver and macrophages, and mediates cholesterol metabolism in an isoform- dependent manner. In the central nervous system, APOE is mainly produced by astrocytes, and transports cholesterol to neurons via APOE receptors, which are members of the low absorbance lipoprotein receptor gene family. This protein is involved in Alzheimer’s disease and cardiovascular disease.

15分钟

Catalog number	Description	Quantity
FEK0663-CAP	96-well plate precoated with anti-Human APOE antibody	1
FEK0663-ST	lyophilized recombinant Human APOE standard	10ng/tube×2
FEK0663-A	Human APOE Antibody A	3ml
FEK1455-B	Human APOE Antibody B	3ml
AR1106-1	Sample Diluent	15ml
AR1106-7	TBS-T Wash Buffer (25x)	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
PLA-SEA	Adhesive Plate Sealers	2

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	7336	30862	101167	7284	33941	97075
Standard deviation	454.83	2129.47	7587.52	590.00	2884.98	8542.60
CV(%)	6.2%	6.9%	7.5%	8.1%	8.5%	8.8%

Concentration(ng/ml)	0	3.12	6.25	12.5	25	50	100	200
O.D.	0.003	0.143	0.256	0.448	0.731	1.041	1.413	1.650

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

酶联免疫双抗夹心法

Cell Culture Supernatants, Serum, Plasma (heparin, EDTA), Saliva, milk and Urine