产品名称
Human Apolipoprotein AI/APOA1 ELISA Kit
指标别名
Apo AI; ApoA I; APOA1; apolipoprotein A I; Apolipoprotein A1
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
3.12 ng/ml - 200 ng/ml
文献引用格式
Human Apolipoprotein AI/APOA1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1456)
背景资料
Apolipoprotein A1 is a protein that in humans is encoded by the APOA1 gene. This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The encoded preproprotein is proteolytically processed to generate the mature protein, which promotes cholesterol efflux from tissues to the liver for excretion, and is a cofactor for lecithin cholesterolacyltransferase (LCAT), an enzyme responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1456-CAP | 96-well plate precoated with anti- human APOA1 antibody | 1 |
EK1456-ST | Lyophilized recombinant human APOA1 standard | 200ng/tube×2 |
EK1456-DA | Biotinylated anti- human APOA1 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 8186 | 19826 | 93780 | 8183 | 19180 | 85400 |
Standard deviation | 376.55 | 1447.29 | 4876.56 | 507.34 | 1515.22 | 5209.4 |
CV(%) | 4.6 | 7.3 | 5.2 | 6.2 | 7.9 | 6.1 |
典型数据示例
Concentration(ng/ml) | 0 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100 | 200 |
O.D. | 0.009 | 0.130 | 0.228 | 0.425 | 0.819 | 1.261 | 1.894 | 2.410 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA) and Urine