产品名称
Human Apolipoprotein AI/APOA1 Quick ELISA Kit
指标别名
Apo AI; ApoA I; APOA1; apolipoprotein A I; Apolipoprotein A1
免疫原
Expression system for standard: E.coli; Immunogen sequence: D25-Q267
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Human Apolipoprotein AI/APOA1 Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK1456)
背景资料
Apolipoprotein A1 is a protein that in humans is encoded by the APOA1 gene. This gene encodes apolipoprotein A-I, which is the major protein component of high absorbance lipoprotein (HDL) in plasma. The encoded preproprotein is proteolytically processed to generate the mature protein, which promotes cholesterol efflux from tissues to the liver for excretion, and is a cofactor for lecithin cholesterolacyltransferase (LCAT), an enzyme responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein.
试剂盒内容
Catalog number | Description | Quantity |
FEK0663-CAP | 96-well plate precoated with anti-Human APOA1 antibody | 1 |
FEK0663-ST | lyophilized recombinant Human APOA1 standard | 10ng/tube×2 |
FEK0663-A | Human APOA1 Antibody A | 3ml |
FEK1456-B | Human APOA1 Antibody B | 3ml |
AR1106-1 | Sample Diluent | 15ml |
AR1106-7 | TBS-T Wash Buffer (25x) | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop Solution | 10ml |
PLA-SEA | Adhesive Plate Sealers | 2 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 8188 | 19829 | 93784 | 8185 | 19184 | 85441 |
Standard deviation | 368.46 | 1249.22 | 5533.25 | 532.02 | 1477.16 | 5382.78 |
CV(%) | 4.5% | 6.3% | 5.9% | 6.5% | 7.7% | 6.3% |
典型数据示例
Concentration(ng/ml) | 0 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100 | 200 |
O.D. | 0.021 | 0.157 | 0.271 | 0.483 | 0.814 | 1.311 | 1.870 | 2.285 |
保存条件与有效期
Store at 4℃ for 6 months.
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA) and Urine