Human Apolipoprotein AI/APOA1 Quick ELISA Kit

96T/3580

Apo AI; ApoA I; APOA1; apolipoprotein A I; Apolipoprotein A1

Expression system for standard: E.coli; Immunogen sequence: D25-Q267

There is no detectable cross-reactivity with other relevant proteins.

<50pg/ml

3.12ng/ml-200ng/ml

APOA1

apolipoprotein A-I

P02647

Human Apolipoprotein AI/APOA1 Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK1456)

Apolipoprotein A1 is a protein that in humans is encoded by the APOA1 gene. This gene encodes apolipoprotein A-I, which is the major protein component of high absorbance lipoprotein (HDL) in plasma. The encoded preproprotein is proteolytically processed to generate the mature protein, which promotes cholesterol efflux from tissues to the liver for excretion, and is a cofactor for lecithin cholesterolacyltransferase (LCAT), an enzyme responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein.

15分钟

Catalog number	Description	Quantity
FEK0663-CAP	96-well plate precoated with anti-Human APOA1 antibody	1
FEK0663-ST	lyophilized recombinant Human APOA1 standard	10ng/tube×2
FEK0663-A	Human APOA1 Antibody A	3ml
FEK1456-B	Human APOA1 Antibody B	3ml
AR1106-1	Sample Diluent	15ml
AR1106-7	TBS-T Wash Buffer (25x)	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
PLA-SEA	Adhesive Plate Sealers	2

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	8188	19829	93784	8185	19184	85441
Standard deviation	368.46	1249.22	5533.25	532.02	1477.16	5382.78
CV(%)	4.5%	6.3%	5.9%	6.5%	7.7%	6.3%

Concentration(ng/ml)	0	3.12	6.25	12.5	25	50	100	200
O.D.	0.021	0.157	0.271	0.483	0.814	1.311	1.870	2.285

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

酶联免疫双抗夹心法

Cell Culture Supernatants, Serum, Plasma (heparin, EDTA) and Urine