产品名称
Human Apolipoprotein AI/APOA1 EZ-Set™ ELISA Kit (DIY Antibody Pairs)
指标别名
Apo AI; ApoA I; APOA1; apolipoprotein A I; Apolipoprotein A1
免疫原
Expression system for standard: E.coli; Immunogen sequence: D25-Q267
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
3.12 ng/ml - 200 ng/ml
文献引用格式
Human Apolipoprotein AI/APOA1 EZ-Set™ ELISA Kit (DIY Antibody Pairs) (Boster Biological Technology, Wuhan, China. Catalog#EZ1456)
背景资料
Apolipoprotein A1 is a protein that in humans is encoded by the APOA1 gene. This gene encodes apolipoprotein A-I, which is the major protein component of high absorbance lipoprotein (HDL) in plasma. The encoded preproprotein is proteolytically processed to generate the mature protein, which promotes cholesterol efflux from tissues to the liver for excretion, and is a cofactor for lecithin cholesterolacyltransferase (LCAT), an enzyme responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein.
试剂盒内容
| Catalog number |
Description |
Quantity |
Storage of opened/reconstituted material |
| EZ1456-CA |
Rabbit anti- human APOA1 polyclonal antibody (Capture Antibody) |
500 µl, 0.4 mg/mL
(recommended dilution 1:100) |
Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit.
|
| EZ1456-DA |
Biotinylated goat anti- human APOA1 polyclonal antibody (Detection Antibody) |
500 µl, 33 µg/mL
(recommended dilution 1:100) |
| AR1103 |
Avidin-Biotin-Peroxidase Complex (ABC) |
500 µl (recommended dilution 1:100) |
| EZ1456-ST |
Lyophilized recombinant human APOA1 standard |
200 ng/tube×3
|
Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the
expiration date of the kit. |
典型数据示例
| Concentration (ng/ml) | 0 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100 | 200 |
| O.D. | 0.009 | 0.13 | 0.228 | 0.425 | 0.819 | 1.261 | 1.894 | 2.41 |
保存条件与有效期
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
需要自备的试剂
1. Microplate reader in standard size.
2. Automated plate washer.
3. Incubator.
4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
5. Clean tubes and Eppendorf tubes.
6. 96 well microplate (Cat# AR1100)
7. Plate Sealers.
8. Capture Antibody Diluent: PBS.
9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
10. Substrate Solution:
Tetramethylbenzidine (Cat# AR1104)
11. Stop Solution: 2 N H2SO4 (Cat# AR1105)
12.Wash Buffer (PBS and PBS-T). Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
*Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)
*样品准备的相关试剂也不含在试剂盒中。
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA) and Urine
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