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Anti-BTK Antibody (Clone#OTI12D4)

Mouse monoclonal antibody

说明书

M00245-3

  • 50μl ¥1180 100μl ¥1960 150μl ¥2780
  • 货期: 5-7天
  • Figure 1. Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-BTK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00245-3, Dilution 1:50)

    all(7)
  • Figure 2. Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-BTK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00245-3, Dilution 1:50)

    all(7)
  • Figure 3. Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-BTK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00245-3, Dilution 1:50)

    all(7)
  • Figure 4. HEK293T cells transfected with either pCMV6-ENTRY BTK (Red) or empty vector control plasmid (Blue) were immunostained with anti-BTK mouse monoclonal (M00245-3), and then analyzed by flow cytometry.

    all(7)
  • Figure 5. Anti-BTK mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTK .

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  • Figure 6. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTK.

    all(7)
  • Figure 7. Western blot analysis of extracts (35ug) from 2 different tissues and Jurkat cells by using anti-BTK monoclonal antibody (1:500).

    all(7)

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产品名称
Anti-BTK Antibody (Clone#OTI12D4)
规格/价格
50μl/1180 100μl/1960 150μl/2780
指标别称
AGMX1; AT; ATK; B cell progenitor kinase; BPK; Bruton tyrosine kinase; BTK; IMD1; PSCTK1; Tyrosine protein kinase BTK; XLA
产品类型
Monoclonal
检验物种
human, mouse, rat
应用范围
WB, IHC, IF, FCM
基因名称
BTK
克隆号
OTI12D4
抗体来源
Mouse
抗体亚型
IgG2a
免疫原
Full length human recombinant protein of human BTK (NP_000052) produced in HEK293T cell.
计算分子量
76 kDa
实际分子量
76 kDa
成分
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
纯化方式
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
浓度
500 ug/ml
产品形态
Liquid
保存条件
Stable for 12 months from date of receipt. Store at -20°C as received.
背景资料
The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes,and associated with a failure of Ig heavy chain rearrangement.
Uniprot ID
Q06187  
文献引用格式
BTK Antibody (Clone#OTI12D4) (Boster Biological Technology, Wuhan, China. Catalog#M00245-3)
应用释义
WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC-免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法
基因名全称
Tyrosine-protein kinase BTK
推荐稀释比
Western blot (WB):1:2000
Immunohistochemistry (IHC):1:50
Immunofluorescence (IF):1:100
Flow cytometry (FCM):1:100

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